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The diagnostic experiences of autistic adults in New Zealand have not been investigated and little is known globally about autistic adults’ satisfaction with the autism diagnostic process. This study describes the diagnostic experiences of 70 autistic adults living in New Zealand and explores how these experiences are related to satisfaction during three stages of the diagnostic process. The results show that autistic adults were reasonably satisfied with the early query and diagnostic assessment stages, but were dissatisfied with the post-diagnostic support stage, with significant unmet needs. Dissatisfaction during the post-diagnostic support stage was also related to satisfaction during previous stages and poor coordination of supports. Suggestions are made on how to improve the autism diagnostic pathway for autistic adults in New Zealand.Many studies have indicated that histone deacetylase inhibitors (HDACis) have a significant antitumor effect in cancer. Here we report a compound named LT-548-133-1 that not only acts as an HDAC inhibitor but also interferes with microtubule assembly to inhibit MCF-7 cell proliferation and induce apoptosis. Consistent with Chidamide, LT-548-133-1 inhibited HDAC activity and increased histone H3 acetylation. But the difference is that it significantly induced cell cycle G2/M arrest while Chidamide caused G0/G1 arrest in MCF-7 cells. By Western blotting, we found the accumulation of CyclinB1 and phosphorylated histone H3 in LT-548-133-1 treated cells. Immunofluorescence based microtubule-repolymerization experiments and immunofluorescence staining of cell microtubules and nuclei showed that LT-548-133-1inhibited microtubule-repolymerization and induced mitotic abnormalities. The decreased expression of Bcl-2 and the increased expression of Bax, p53, p21, and cleaved-Caspase3 indicated the occurrence of apoptosis. Flow cytometry results also showed an increase in the proportion of apoptotic cells after administration of LT-548-133-1 or Chidamide. Therefore, we demonstrated that LT-548-133-1 could act as an HDAC inhibitor while inhibiting microtubule-repolymerization, causing mitosis to be arrested in G2/M. These two effects ultimately lead to proliferation inhibition and apoptosis of MCF-7 cells.

To determine the safety, efficacy and clinical outcomes of 100µ versus 160µ cap in patients undergoing ReLEx-Small Incision Lenticule Extraction (SMILE).

This prospective, comparative, non-randomized clinical trial included hundred eyes from 50 patients, undergoing bilateral ReLEx SMILE for myopia ranging from -1 to -6 D spherical equivalent. Twenty-five patients received treatment with standard 100µ cap thickness, while the remaining 25 patients underwent the same procedure but with a 160µ cap thickness in both eyes. Manifest refraction, UDVA, CDVA, contrast sensitivity, aberrations and dry eye were evaluated along with a subjective questionnaire at 2weeks and 3months post-operatively. Mean follow-up was 90 ± 15days.

At two weeks and 3months post-operative, compared to the preoperative values, the mean log-MAR UDVA, CDVA, spherical equivalent, contrast sensitivity higher order aberrations did not show statistically significant differences (p > .05) between the two study groups. However, significant reduction in Schirmer’s II and TBUT scores was observed compared to preoperative scores at 3months in 100µ group (p > .05) but not in160µ group. Patients did not report significant subjective complaints in either groups treated, when leading questions were asked through a subjective questionnaire.

ReLEx SMILE with 160µ cap thickness was equally safe and efficacious as 100 µ cap, with no unique complications observed by keeping a thicker cap. Post-operative dry eye was significantly less in 160 µ group, suggesting an advantage in patients with pre existing dryness or contact lens users.

CTRI/2014/09/005,005.

CTRI/2014/09/005,005.

To evaluate ocular surface changes among patients with psoriasis in Malaysia, a developing country in Southeast Asia.

An interdisciplinary case-control study (60 psoriasis patients and 40 control subjects) to look at the differences in ocular surface manifestations between patients with psoriasis and a group of age-, gender- and ethnicity-matched healthy controls.

One hundred and twenty eyes of 60 patients with psoriasis and 80 eyes of 40 healthy controls without psoriasis were included in the study. Mild-to-moderate psoriasis was found in 42 patients (70%), while 18 patients (30%) had severe psoriasis. Psoriatic arthritis was found in 19 patients (32%). Of the 60 psoriatic patients, the prevalence of ocular involvement was 65% (39/60), in which 32% (19/60) had dry eyes, 27% (16/60) had lid margin abnormalities, 33% (20/60) had cataract, and one had history of anterior uveitis. Compared to controls, ocular surface of psoriatic patients showed more eyelid margin abnormalities, higher meibomian gland loss and lower tear filmbreak-up time. The estimated odds ratio for dry eyes in the psoriasis group was 2.2 (95% CI 0.8-6.9).

Ocular surface disorders encompassing eyelid margin abnormalities, meibomian gland loss and tear dysfunction occur at an earlier and higher rate among psoriatic patients.

Ocular surface disorders encompassing eyelid margin abnormalities, meibomian gland loss and tear dysfunction occur at an earlier and higher rate among psoriatic patients.COVID-19 is an ongoing worldwide infectious disease pandemic. The purpose of this study was to investigate post-traumatic stress and related factors among hospital nurses during the COVID-19 outbreak. The subjects of this study were 300 nurses who worked in three general hospitals that operated National Designated Isolation Unit (NDIU) wards during the COVID-19 outbreak. check details Self-reporting questionnaires were used to collect data on post-traumatic stress, general characteristics, and work-related information. The average post-traumatic stress score was 20.68 ± 19.5 points and 36.7% of participants were at high risk of post-traumatic stress disorder (PTSD). The odds ratio (OR) for PTSD was higher for nurses who worked in the NDIU ward (OR = 16.31, 95% CI = 3.79-70.32), who responded that nurse staffing was poor (OR = 3.03, 95% CI = 1.01-9.10), and who responded that they experienced COVID-19 symptoms (OR = 3.83, 95% CI = 1.89-7.75). Total 36.7% of nurses were at risk of PTSD and the factors related to PTSD were the work department, nurse staffing, and experiencing COVID-19 symptoms.