Activity

In the liver, due to higher basal antioxidant protection, limited effects were observed. Genotoxic damage increased under the combined stressors scenario. Overall results highlighted the modulation of the oxidative stress response to Cd by multiple stressors, suggesting the vulnerability of T. bernacchii under predicted ocean change scenarios.Palmitic acid (PA), a saturated fatty acid enriched in high-fat diet, has been implicated in the development of sarcopenic obesity. Herein, we chose two non-cytotoxic concentrations to better understand how excess PA could impact myotube formation or diameter without inducing cell death. Forty-eight hours of 100 µM PA induced a reduction of myotube diameter and increased the number of type I fibers, which was associated with increased miR-206 expression. Next, C2C12 myotube growth in the presence of PA was evaluated. Compared to control cells, 150 µM PA reduces myoblast proliferation and the expression of MyoD and miR-206 and miR-133a expression, leading to a reduced number and diameter of myotubes. PA (100 µM), despite not affecting proliferation, impairs myotube formation by reducing the expression of Myf5 and miR-206 and decreasing protein synthesis. Interestingly, 100 and 150 µM PA-treated myotubes had a higher number of type II fibers than control cells. In conclusion, PA affects negatively myotube diameter, fusion, and metabolism, which may be related to myomiRs. MK4827 By providing new insights into the mechanisms by which PA affects negatively skeletal muscle, our data may help in the discovery of new targets to treat sarcopenic obesity.The objective of this study was to describe and compare the fecal bacterial microbiota of horses with equine recurrent uveitis (ERU) and healthy horses using next-generation sequencing techniques. Fecal samples were collected from 15 client-owned horses previously diagnosed with ERU on complete ophthalmic examination. For each fecal sample obtained from a horse with ERU, a sample was collected from an environmentally matched healthy control with no evidence of ocular disease. The Illumina MiSeq sequencer was used for high-throughput sequencing of the V4 region of the 16S rRNA gene. The relative abundance of predominant taxa, and alpha and beta diversity indices were calculated and compared between groups. The phyla Firmicutes, Bacteroidetes, Verrucomicrobia, and Proteobacteria predominated in both ERU and control horses, accounting for greater than 60% of sequences. Based on linear discriminant analysis effect size (LEfSe), no taxa were found to be enriched in either group. No significant differences were observed in alpha and beta diversity indices between groups (p > 0.05 for all tests). Equine recurrent uveitis is not associated with alteration of the gastrointestinal bacterial microbiota when compared with healthy controls.Organic solvent nanofiltration (OSN) has been recognized as an eco-friendly separation system owing to its excellent cost and energy saving efficiency, easy scale-up in the narrow area and mild operation conditions. Membrane properties are the key part in terms of determining the separation efficiency in the OSN system. In this review paper, the recently reported OSN thin-film composite (TFC) membranes were investigated to understand insight of membrane materials and performance. Especially, we highlighted the representative study concepts and materials of the selective layer of OSN TFC membranes for non-polar solvents. The proper choice of monomers and additives for the selective layer forms much more interconnected voids and the enhanced microporosity, which can improve membrane performance of the OSN TFC membrane with reducing the transport resistance. Therefore, this review paper could be an important bridge to connect with the next-generation OSN TFC membranes for non-polar solvents.Understanding people’s eating habits plays a crucial role in interventions promoting a healthy lifestyle. This requires objective measurement of the time at which a meal takes place, the duration of the meal, and what the individual eats. Smartwatches and similar wrist-worn devices are an emerging technology that offers the possibility of practical and real-time eating monitoring in an unobtrusive, accessible, and affordable way. To this end, we present a novel approach for the detection of eating segments with a wrist-worn device and fusion of deep and classical machine learning. It integrates a novel data selection method to create the training dataset, and a method that incorporates knowledge from raw and virtual sensor modalities for training with highly imbalanced datasets. The proposed method was evaluated using data from 12 subjects recorded in the wild, without any restriction about the type of meals that could be consumed, the cutlery used for the meal, or the location where the meal took place. The recordings consist of data from accelerometer and gyroscope sensors. The experiments show that our method for detection of eating segments achieves precision of 0.85, recall of 0.81, and F1-score of 0.82 in a person-independent manner. The results obtained in this study indicate that reliable eating detection using in the wild recorded data is possible with the use of wearable sensors on the wrist.Griscelli syndrome (GS) is a rare disease that is characterized by silvery hair and fair skin. It is included in congenital grey hair syndromes, a rare group of autosomal recessive disorders characterized by silvery grey hair and severe multisystem disorders, such as immune system impairment, defects in immunological function, ocular and skeletal alterations, and nervous system defects. Herein, we report a rare case of GS type 1 and highlight the importance of a dermatological and hair examination to make an early diagnosis of these life-threatening diseases.(1) School-based physical activity programs such as intramurals provide youth with inclusive opportunities to be physically active, yet we know little about how types of intramurals (e.g., team and individual sports) may contribute to youth MVPA. This research aims to evaluate how real-world changes in types of intramurals available in schools impact youth physical activity over time. (2) This study used three years of longitudinal school- and student-level data from Ontario schools participating in year 5 (2016-2017), year 6 (2017-2018) and year 7 (2018-2019) of the COMPASS study. Data on types of intramural programs from 55 schools were obtained, baseline demographic characteristics were measured and data on physical activity and sport participation were collected on a sample of 4417 students. Hierarchical linear mixed regression models were used to estimate how changes in the type of intramurals associate with youth MVPA over time. (3) Regardless of participation, adding individual and team intramurals was significantly and positively associated with female MVPA in Y6.