Activity

Computational protein design can generate proteins not found in nature that adopt desired structures and perform novel functions. Although proteins could, in theory, be designed with ab initio methods, practical success has come from using large amounts of data that describe the sequences, structures, and functions of existing proteins and their variants. We present recent creative uses of multiple-sequence alignments, protein structures, and high-throughput functional assays in computational protein design. Approaches range from enhancing structure-based design with experimental data to building regression models to training deep neural nets that generate novel sequences. Looking ahead, deep learning will be increasingly important for maximizing the value of data for protein design.

To compare the anatomical and audiological outcomes of endoscopic type I tympanoplasty using cartilage-perichondrium, with or without a customized 3D-printed guiding template.

A total of 60 patients with tympanic membrane perforation receiving endoscopic type I tympanoplasty were divided into the non-template group (group 1, n=30) and template group (group 2, n=30). Closure rate, hearing outcomes and operating time were compared between the two groups.

Group1 had a significant higher operation time compared with group2 (77.73±10.63min vs. 66.23±14.92min, p=0.001). this website The overall closure rate of group1 was lower than that of group2 (83.33% vs. 100%, p=0.052). The postoperative air-bone gaps (ABGs) were significantly lower than preoperative ones in each group (p<0.001, respectively).

Improvements in hearing outcomes were comparable for the two groups. The applying of customized 3D-printed guiding template resulted in a higher closure rate and a shorter operation time. Our results suggest that the customized 3D-printed guiding template can be recommended as a useful aid for endoscopic type I tympanoplasty.

Improvements in hearing outcomes were comparable for the two groups. The applying of customized 3D-printed guiding template resulted in a higher closure rate and a shorter operation time. Our results suggest that the customized 3D-printed guiding template can be recommended as a useful aid for endoscopic type I tympanoplasty.Graft-versus-host disease (GVHD) is a common complication that increases morbidity and mortality after allogeneic stem cell transplantation (allo-SCT). Fluorodeoxyglucose F 18 (18F-FDG)-positron emission tomography (PET) imaging has been demonstrated to be highly informative for evaluating and mapping of intestinal GVHD. To corroborate and extend existing findings and to investigate whether glucose metabolism assessed by 18F-FDG-PET might be an effective diagnostic tool to predict corticosteroid-refractory acute GVHD and overall survival. In this retrospective analysis, 101 patients with clinically suspected acute intestinal GVHD underwent 18F-FDG-PET between June 2011 and February 2019. Seventy-four of these patients with clinically and/or histologically proven acute intestinal GVHD as well as positive 18F-FDG-PET findings were analyzed in detail to assess the predictive value of 18F-FDG-PET regarding the response to immunosuppressive therapy and survival. Quantitative PET parameters, particularly the maximuh a median OS of 390 versus 117 days for patients with SUVmax ≤8.95 (P = .036). SUVmax threshold and donor type were independent factors for OS. Our results indicate that 18F-FDG-PET is highly accurate in identifying patients with acute intestinal GVHD and may predict responses to immunosuppressive therapy as well as survival, particularly when applied within the first 100 days after transplantation. These results provide a strong rationale to integrate PET imaging in future prospective trials evaluating new therapies for acute GVHD.

Most previous studies reported biomechanical deficits in individuals with a trans-tibial amputation (TTA) during gait using zero-dimensional analyses. However, these analyses do not allow to precisely determine during which part of the gait cycle these deficits occur. There is a need to use more appropriate methods to map the differences, such as one-dimensional statistical parametric mapping.

What are the most relevant phases of the gait cycle during which the biomechanical deficits in TTA occur?

Eight TTA and 15 healthy counterparts (CON) underwent one biomechanical gait analysis. Pelvis, hip, knee and ankle kinematics, total support moment (TSM) and gastrocnemius lateralis, vastus lateralis and tibialis anterior muscle activity were compared between the amputated (AmLL), the intact (InLL) and the control (CnLL) lower limbs using one-dimensional statistical parametric mapping.

More ankle dorsiflexion and knee flexion were observed for the AmLL compared to the InLL and CnLL (ankle only) from the end of the stance phase to the beginning of the swing phase. Less knee flexion was also found for the AmLL during early stance phase. More pelvis posterior tilt and rotation toward the contralateral limb was observed during most of the gait cycle for the AmLL compared to the InLL. TSM was smaller for the AmLL compared to the CnLL during early stance phase.

Using a one-dimensional statistical parametric mapping approach for TTA gait analysis, this study provides novel insights on their biomechanical gait deficits compared to CON. Greater reliance on the InLL was observed in TTA as suggested by the asymmetric kinematic and kinetic profiles.

Using a one-dimensional statistical parametric mapping approach for TTA gait analysis, this study provides novel insights on their biomechanical gait deficits compared to CON. Greater reliance on the InLL was observed in TTA as suggested by the asymmetric kinematic and kinetic profiles.Insect diapause shares many biochemical features with other states of metabolic depression, including the suppression of global metabolism, reorganization of metabolic pathways and improved stress resistance. However, little is known about the biochemical changes associated with the diapause phenotype in tropical insects. To investigate biochemical adaptations associated with tropical diapause, we measured the activities of metabolic and antioxidant enzymes, as well as glutathione levels, in the sunflower caterpillar Chlosyne lacinia at different times after initiation of diapause ( less then 1, 20, 40, 60, and 120 days) and after arousal from diapause. Biochemical changes occurred early in diapausing animals, between the first 24 h and 20 days of diapause. Diapausing animals had reduced oxidative capacity associated with a decrease in the activities of peroxide-decomposing antioxidant enzymes. There was no sign of redox imbalance either during diapause or after recovery from diapause. Noteworthy, glutathione transferase and isocitrate dehydrogenase-NADP+ activities sharply increased in diapausing animals and stand out as diapause-associated proteins.