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This is in line with recent findings that it is mutated by lysine (E484K mutation) in the rapidly spreading variants of COVID-19 belonging to the B.1.351 and P.1 lineages. In addition, this mutation is reported to cause virus neutralization escapes from highly neutralizing COVID-19 convalescent plasma. Thus, further efforts are required to probe its functional relevance. Overall, our results present a systematic understanding of the energetic binding features of SARS-CoV-2 RBD with the ACE2 receptor, which can provide a valuable insight for the design of SARS-CoV-2 drugs and identification of cross-active antibodies.Although unsaturated organotrifluoroborates are common synthons in metal-organic chemistry, their transition metal complexes have received little attention. [CH2(3,5-(CH3)2Pz)2]Cu(CH2[double bond, length as m-dash]CHBF3), (SIPr)Cu(MeCN)(CH2[double bond, length as m-dash]CHBF3) and [CH2(3,5-(CH3)2Pz)2]Ag(CH2[double bond, length as m-dash]CHBF3) represent rare, isolable molecules featuring a vinyltrifluoroborate ligand on coinage metals. The X-ray crystal structures show the presence of three-coordinate metal sites in these complexes. The vinyltrifluoroborate group binds asymmetrically to the metal site in [CH2(3,5-(CH3)2Pz)2]M(CH2[double bond, length as m-dash]CHBF3) (M = Cu, Ag) with relatively closer M-C(H)2 distances. The computed structures of [CH2(3,5-(CH3)2Pz)2]M(CH2[double bond, length as m-dash]CHBF3) and M(CH2[double bond, length as m-dash]CHBF3), however, have shorter M-C(H)BF3 distances than M-C(H)2. These molecules feature various inter- or intra-molecular contacts involving fluorine of the BF3 group, possibly affecting these M-C distances. The binding energies of [CH2[double bond, length as m-dash]CHBF3]- to Cu+, Ag+ and Au+ have been calculated at the wB97XD/def2-TZVP level of theory, in the presence and absence of the supporting ligand CH2(3,5-(CH3)2Pz)2. The calculation shows that Au+ has the strongest binding to the [CH2[double bond, length as m-dash]CHBF3]- ligand, followed by Cu+ and Ag+, irrespective of the presence of the supporting ligand. However, in all three metals, the supporting ligand weakens the binding of olefin to the metal. The same trends were also found from the analysis of the σ-donation and π-backbonding interactions between the metal fragment and the π and π* orbitals of [CH2[double bond, length as m-dash]CHBF3]-.The change in hyaluronidase (HAase) is related to specific changes in the structure of vitreous, and it is necessary to develop simple but sensitive methods for HAase detection. In this work, a thin film fabricated from a hyaluronic acid (HA)-polyethyleneimine (PEI) hydrogel has been covered on a mixed cellulose microporous membrane (MCEM) to form a HA-PEI-MCEM firstly and it was then applied in a filtration system. The permeability of the filter membrane greatly affects the amount of water passing through within a certain time and the water can be collected and quantitatively measured with a simple electronic balance easily. The low permeability of the HA-PEI-MCEM allows a small amount of water to be drained. But after the addition of HAase, which can hydrolyze HA in the hydrogel, the permeability of the membrane increased. Therefore, the amount of water passing through the HA-PEI-MCEM composite membrane increased accordingly. The composite of the membrane, and the reaction conditions after the addition of HAase were optimized. Under the optimized conditions, the amount of water collected within 5 min showed a linear relationship with the HAase concentration in a range of 1.0-36 U mL-1 with a limit of detection of 0.35 U mL-1.The proposed method has been applied to detect HAase in vitreous samples with satisfactory results.Biological and pharmaceutical analytes like liposomes, therapeutic proteins, nanoparticles, and drug-delivery systems are utilized in applications, such as pharmaceutical formulations or biomimetic models, in which controlling their size is often critical. Many of the common techniques for sizing these analytes require method development, significant sample preparation, large sample quantities, and lengthy analysis times. In other cases, such as DLS, sizing can be biased towards the largest constituents in a mixture. Therefore, there is a need for more rapid, sensitive, accurate, and straightforward analytical methods for sizing macromolecules, especially those of biological origin which may be sample-limited. Taylor dispersion analysis (TDA) is a sizing technique that requires no calibration and consumes only nL to pL sample volumes. In TDA, average diffusion coefficients are determined via the Taylor-Aris equation by characterizing band broadening of an analyte plug under well-controlled laminar flow condit and materials science.High-resolution live imaging promises new insights into the cellular and molecular dynamics of the plant root system in response to external cues. Microfluidic platforms are valuable analytical tools that combine the precise spatial and temporal control of culture conditions with live-imaging capabilities. learn more However, complexity in the fabrication and operations of current plant microfluidic platforms limits their use to a few technologically-focused laboratories. Here, we design and characterize an easy-to-implement 3D printed open microfluidic platform for Arabidopsis thaliana roots. Our biocompatibility study identified a suitable material for the platform production and an established drought stress assay validates the reliability of our stereolithography (SLA)-based next generation RootChip.Chemical warfare agents (CWAs) are toxic chemicals that have been used as disabling or lethal weapons in war, terrorist attacks, and assasinations. The Chemical Weapons Convention (CWC) has prohibited the use, development, production, and stockpiling of CWAs since its initiation in 1997, however, the threat of deployment still looms. Detection of trace CWAs post-deployment or post-remediation, in bulk matrices such as soil, often requires lengthy sample preparation steps or extensive chromatographic separation times. 3D-printed cone spray ionization (3D-PCSI), an ambient ionization mass spectrometric (MS) technique, provides a rapid, simple, and low-cost method for trace CWA analysis in soil matrices for both in-laboratory and in-field detection. Described here is the utilization of conductive 3D-printed cones to perform both rapid sampling and ionization for CWA simulants and hydrolysis products in eight solid matrices. The analysis of trace quantities of CWA simulants and hydrolysis products by 3D-PCSI-MS coupled to both a commercial benchtop system and a field-portable MS system is detailed.