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Specifically, micronutrients such as magnesium, phosphorous, zinc, potassium, and iron were increased while others showed rise in fibre and protein levels. The fortification of staple foods with UUPS has been shown to be promising; however, more designed feeding trials are required to verify the impact on reducing undernutrition and hidden hunger. To do this, it is recommended that rice fortified with UUPS should be targeted as rice is increasingly becoming the leading and important staple food in Africa.Dermatophytosis is a common contagious disease of both humans and animals. It is caused by a group of filamentous fungi known as dermatophytes, including several genera and various species. An accurate diagnosis of dermatophytes as a causative agent of a skin lesion requires up to one month of conventional laboratory diagnostics. The conventional gold standard diagnostic method is a direct microscopic examination followed by 3 to 4 weeks of Sabouraud’s dextrose agar (SDA) culturing, and it may require further post-culturing identification through biochemical tests or microculture technique application. Y-27632 The laborious, exhaustive, and time-consuming gold standard method was a real challenge facing all dermatologists to achieve a rapid, accurate dermatophytosis diagnosis. Various studies developed more rapid, accurate, reliable, sensitive, and specific diagnostic tools. All developed techniques showed more rapidity than the classical method but variable specificities and sensitivities. An extensive bibliography is included and discussed through this review, showing recent variable dermatophytes diagnostic categories with an illustration of weaknesses, strengths, and prospects.Reproductive diseases may have destructive effects on the fertility of cattle. Bovine viral diarrhoea virus (BVDV) and bovine herpes virus-1 (BoHV-1) are potent viral pathogens linked to reproduction. Thus, the aim of this study was to utilize raw semen samples for conventional and molecular detection of BVDV and BoHV-1, simultaneously. Additionally, the effect of virus infection on the semen quality of naturally infected bulls has been investigated. Therefore, 40 bulls were employed for semen collection, evaluation and testing for both viruses by virus isolation, direct fluorescent antibody technique (FAT) and SYBR Green real-time PCR assay. In virus isolation results, no cytopathic effect (CPE) was observed for BVDV on cell culture whereas, eight (20%) samples displayed characteristic grape-like clusters of cells for BoHV-1. By direct FAT, 12 (30%) positive BVDV and 8 (20%) positive BoHV-1 samples were confirmed. SYBR Green real-time PCR analysis using 48 h inoculated semen samples revealed 14 (35%) and 8 (20%) positive samples for BVDV and BoHV-1, respectively. Statistical analysis of semen evaluation parameters showed a significant difference between viral-infected and free groups represented by increased sperm abnormalities and decreased sperm motility, liveability and concentration. However, there was no significant difference among BVDV, BoHV-1 and mixed-infected groups. The study concluded that BVDV and/or BoHV- 1 infected bulls expressed low semen quality. Real-time PCR was confirmed to be the ideal laboratory assay for detection of both viruses in semen.In ruminants, the rumen is the largest and most significant fore-stomach. Stereological analysis of important structural parameters that may be used to assess the functional capacity of the rumen is lacking. In the current investigation, five rams were used to demonstrate the methods for quantifying salient structural parameters related to rumen function. The sheep were euthanized with 20% sodium pentobarbital intravenously, the rumen was dissected out and divided into the various sacs for gross examination, and fixation by total immersion in 10% formalin. Macroscopic ruminal surface area was estimated using the point-associated area method. Volumes of the ruminal tissues were estimated by the volume displacement method, while volume densities of the components of the ruminal wall were estimated by point counting methods. Tissue blocks for histology were obtained by systematic random sampling and processed to obtain vertical sections for surface area and volume estimations. Papillary densities and numbers were estimated from horizontal sections. The volume of ruminal tissue was 536.54 ± 80.52 cm3, the macroscopic surface area was 1091 ± 115.75 cm2 with a papillary packing density of 84.64 ± 10.99 cm-2. Average absolute surface area was 4726.74 ± 628.56 cm2. The total number of ruminal papillae per rumen was 92,884.91 ± 6216.46. The methods documented here provide the possibility of doing a detailed stereological analysis of ruminal tissue in different experimental or even pathological conditions.We present a case of a 71-year-old female with complete heart block and an incidentally found atrioventricular nodal inclusion cyst.Paravalvular leaks (PVL) are seen in 5-17% of patients after surgical mitral and aortic valve replacement. This is usually well-tolerated in the majority of patients; however, up to 5% will require re-intervention due to either hemodynamically significant regurgitation or hemolysis requiring repeated blood transfusion. Transcatheter closure of PVLs is becoming the treatment of choice in many patients owing to the high risk of redo surgery, high rates of recurrence with the surgical approach, and substantial improvements in device technology and growing expertise in structural heart disease interventions. Careful selection of the appropriate candidates by the Heart Team with in-depth analysis of clinical and multimodality imaging data is critical to ensuring good short- and long-term outcomes1. The defect is usually oval/crescentic and often serpiginous in nature, which poses significant challenges in choosing the optimal size and number of devices to implant – especially with large size defects. Generally, defects involving more than 25-30% of the sewing ring are deemed unsuitable for percutaneous closure. While the Amplatzer family of vascular plugs (e.g. AVP3 and AVP2) is commonly used for percutaneous closure of PVLs, there are currently no approved dedicated devices for this indication, except the paravalvular leak device (Occlutech) which is not universally available. Small and relatively circular defects can usually be closed using a single plug, conventionally utilizing a size that is 25-30% larger than the mean diameter of the defect. Larger and crescentic defects on the other hand frequently require more than one plug and can be quite challenging in terms of choosing the appropriate size(s)2. We report two cases with very large defects with irregular shape in which 3D printed modeling was extremely useful for bench testing to optimize the number and sizes of devices to be implanted.