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gondii by wild boar; therefore, consumption of raw or undercooked game meat of infected animals can carry a significant risk of T. gondii infection.INTRODUCTION A new monogenean, Heterocotyle margaritae n. sp. (Monocotylidae Heterocotylinae), is described based on specimens collected from the gills of the diamond stingray, Hypanus dipterurus (Jordan & Gilbert, 1880) (Myliobatiformes Dasyatidae) captured off the South Pacific coast, Lima Region, Peru. MATERIALS AND METHODS Monogeneans were fixed in hot 4% formalin, observed and measured as permanent mounts stained with Gomori’s trichrome, and mounted in Canada balsam. Drawings were made with the aid of a drawing tube. RESULTS The new species is distinguished from all congeners mainly by having a funnel-shaped male copulatory organ, spatulate distally with lateral folds and by its club-shaped accessory piece. The most similar congener, H. americana Hargis, 1955, resemble H. margaritae n. sp. regarding the general structure of the male copulatory organ (funnel-shaped with an accessory piece). However, besides other features, the new species differ from H. americana by having an accessory piece without a dorsal button (vs an accessory piece with a dorsal button in H. americana). CONCLUSIONS This is the first report of a member of Heterocotyle Scott, 1904 in the Southeastern Pacific Ocean. The present finding brings to 20 the number of known species of Heterocotyle, and represents the fifth described monogenean species from Hypanus dipterurus.INTRODUCTION A new coccidian species of the genus Eimeria Schneider, 1875 (Apicomplexa Eimeriidae), is reported from the bat host Myotis riparius Handley from Ilha Grande, a large island off the coast of the State of Rio de Janeiro, in southeastern Brazil. METHODS Bats were captured in 13 mist nets (10 × 3 m), which were set within the experimental plots, and through active searches of the daytime roosts of Molossus molossus Pallas found in Vila Dois Rios. Containment was made in bags for the collection of feces and identification of coccidia. A survey was conducted on the coccidia species described so far (Table 2). RESULTS The oöcysts of Eimeria riparii n. sp. are ellipsoidal to cylindroidal with an extremely thin, bi-layered wall, slightly rough. Two polar granules are present, micropyle and oöcyst residuum are both absent. The sporocysts are ellipsoidal, the sporocyst residuum is formed by sparse, rounded granules of varying sizes; the Stieda body is trapezoidal and a sub-Stieda body is absent. Sporozoites are banana shaped. With the new species described here, a total of 40 Eimeria spp. have been described infecting bat hosts, belonging to 30 species of 18 genera and 5 families. CONCLUSION The subsequent increase in the known diversity of bats has been derived from the ongoing expansion of research in a number of different areas of taxonomy and ecology although the number of studies of the associated coccidian parasites of the family Eimeriidae has increased more slowly.PURPOSE This study aimed to determine the molecular characterization and subtype distribution of Blastocystis sp. isolated from cancer children and adolescents in Shiraz, Fars province, southwestern Iran. METHODS Overall, 200 fecal samples obtained from cancer children and adolescents under 18 years old (107 males and 93 females) and checked by microscopy, culture, and molecular methods (PCR). Possible etiological factors and clinical characteristics of Blastocystis infection were also evaluated and compared between Blastocystis infected and non-infected patients. RESULTS Thirteen of 200 (6.5%) stool samples were positive for Blastocystis by microscopy. While 21 of 200 (10.5%) were positive by culture, and 24 of 200 (12%) were positive by PCR. Out of 24 positive samples tested by PCR and sequencing, ST3 was reported as the most common subtype (nine samples, 37.5%), followed by ST2 (eight samples, 33.3%), ST1 (five samples, 20.9%), and ST7 (two samples, 8.3%). The prevalence of Blastocystis infection in males was significantly higher than females (p = 0.024). Also, Blastocystis was more prevalent in patients who had received at least eight chemotherapy cycles than fewer (p = 0.002). However, no associations were found between Blastocystis-positive rate and age, residence, type of cancers, or contact with animals. Also, there was no significant difference between frequency of Blastocystis subtypes in symptomatic and asymptomatic cancer patients. CONCLUSIONS Various controlled epidemiologic and topographic studies need to confirm or reject these possible associations with Blastocystis infection. The data from this study are an invaluable addition to the growing body of research studies on Blastocystis infection in cancer patients.OBJECTIVES During mitral annuloplasty, we twisted the harvested auto-pericardium to enable adequate ring sizing and implanted it to prevent ring-related complications. Indication for twisted auto-pericardial band (APB) was limited to patients with less than severe annular dilation to ensure high reproducibility and durability. The aim of this study was to investigate the long-term outcomes of twisted APB annuloplasty. METHODS From 1999 to 2009, 107 patients (62 ± 12 years, degenerative 92 and infective endocarditis 15) with isolated posterior mitral leaflet (PML) prolapse with inter-commissural distance of 32 mm or smaller underwent twisted APB annuloplasty. Of these, 104 patients without early leaflet repair failure were studied. Leaflet repairs were predominantly performed by quadrangular resection. Median APB size was 28 mm. RESULTS Follow-up rate was 98.1% and duration was 10.9 ± 4.8 years. There were two early and 34 late deaths. Survival and freedom from cardiac-related death at 15 years were 61.7% ± 5.6% and 83.8% ± 4.7%, respectively. Age and male sex were independent predictors of mortality. There were four late reoperations for recurrent severe mitral regurgitation (MR), three of which were due to new chordal rupture. Freedom from reoperation and freedom from moderate or severe MR at 15 years were 93.1% ± 3.5% and 81.3% ± 6.2%, respectively. No patients developed hemolysis, ring dehiscence, or infective endocarditis. CONCLUSIONS The long-term outcomes of twisted APB for isolated PML prolapse without severe annular dilation are satisfactory. this website Twisted APB annuloplasty may be a preferable option to reduce ring-related complications with sufficient durability.