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While further studies are needed, these results show careful CPA loading and higher metal forms warming rates can help reduce CPA loading toxicity and improve outcomes from cryopreservation in tissues while also offering new protocols to preserve larger tissues ≥1 mm in thickness.The present pandemic has hurled organizations into a new social “norm”-and academia is not exempt. As a result, a novel pedagogy has evolved, demanding mechanisms of social distance teaching and learning be developed. This new era in education has learning institutions acquiring, adapting or even designing online STEM labs for physical science courses-replacing face-to-face teaching laboratory settings. The difficulty of designing science practicum that promotes student engagement and relates scientific principles to “real life” has heightened as we shift to more online instruction. Therefore, a necessity to develop contemporary methods of promoting student engagement and participation in an online learning environment has become apparent. Here, a virtual lab course designed for health science majors, utilized an online lab module and, with the use of a learning management system, adapted it to illustrate the principles of specific heat capacity in “real life” hot coffee. Students enrolled in the lab course were able to perform a virtual lab, complete calculations, compare calculated and experimental values, and relate the experimental chemistry topics to healthcare in a lab report. Following the lab experience, students completed an anonymous and voluntary qualitative survey in which they recommended this lab exercise for future classes.

The ongoing coronavirus disease 2019 (COVID-19) outbreak has elicited concerns about public fear and economic fallout. The current study takes a person-oriented approach to identify the unique response patterns that underlie three risk perception components (likelihood, severity, and protection efficacy) of COVID-19, with information sources as precursors and economic confidence as outcomes.

A total of 1,074 Chinese citizens participated in a national online survey in early February 2020.

A latent profile analysis showed that participants exhibited one of three classes Risk Neutrals (49.9%; moderate in all components), Risk Deniers (14.3%; low likelihood, low severity, and high protection efficacy), or Risk Exaggerators (35.8%; high likelihood, high severity, and low protection efficacy). Subsequent analyses showed that reliance on unofficial sources (gossip and news spread among friends; WeChat) positively correlated with membership in the Risk Exaggerators class. In turn, belonging in the Risk Exaggerators class correlated with the lowest short-term (but not long-term) economic confidence.

This study suggests that exploring the heterogeneity of the public risk perception might help the government to design differentiated risk communication strategies during the COVID-19 outbreak.

This study suggests that exploring the heterogeneity of the public risk perception might help the government to design differentiated risk communication strategies during the COVID-19 outbreak.

High expression of monoamine oxidase A (MAOA) in non-small cell lung cancer (NSCLC) is related to epithelial-mesenchymal transition (EMT) and the development of clinicopathological features of NSCLC. Nevertheless, the role of MAOA in drug resistance still remains unclear. Hence, the aim of this article was to evaluate a previously synthesized MAOA inhibitor (G11) on inhibiting paclitaxel-resistant NSCLC metastasis and growth.

First, MAOA expression level was evaluated in several NSCLC cell lines. An MTT assay was used to validate the inhibitory effect of G11 on NSCLC cells in vitro. Second, gene expression in G11-treated H460/PTX cells was analyzed by microarray gene expression. Third, transwell assay was performed to assess the invasion and metastasis of G11-treated A549/PTX and H460/PTX cells and western blot assay used to analyze vital protein expression level in G11-treated H460/PTX cells. Finally, the antimetastatic effect of G11 was tested in an NSCLC in vivo model.

Our data revealed that G11 signsing target for the treatment of drug-resistant NSCLC.

SIGNIFICANT FINDINGS OF THE STUDY Inhibition of MAOA might contribute to the suppression of metastasis and growth in PTX-resistant NSCLC cells. What this study adds This study explored the potential function of MAOA in drug-resistant NSCLC and might consider MAOA as a promising target for the treatment of drug-resistant NSCLC.

Perianal Paget’s disease is associated with adenocarcinoma and can spread to the perianal skin. It often requires extensive resection of the perianal skin and rectum. Many studies have shown the efficacy of laparoscopic abdominoperineal resection for lower rectal cancer. However, extensive resection of the dorsal side of the perineal skin is difficult in the lithotomy position. We report a laparoscopic abdominosacral approach using the jackknife position for perianal Paget’s disease.

Surgery was started using the lithotomy position, and total mesorectal excision with central lymphadenectomy was performed laparoscopically. 5-Chloro-2′-deoxyuridine Pelvic floor muscles were divided 2 cm away from the rectum. The sigmoid colon was then divided with a linear stapler, and a terminal colostomy was made. The sacral approach was then followed with the patient placed in a jackknife position. A skin incision was made 1-2 cm from the negative margin confirmed by preoperative mapping biopsy and resected en-bloc. We used this approach in two patients with a mean operative time of 483 minutes, including 53.5 minutes for the position change. All tumor margins, including the skin, were cancer-free, and primary wound closure was possible in both of the cases. Both patients were doing well without any recurrence 10 and 13 months postoperatively.

Laparoscopic abdominosacral resection is safe and effective and facilitates extensive perineal skin resection, especially on the dorsal side, with a less invasive laparoscopic procedure.

Laparoscopic abdominosacral resection is safe and effective and facilitates extensive perineal skin resection, especially on the dorsal side, with a less invasive laparoscopic procedure.