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In contrast, the protein concentration of TGF-β, BMP-2 and PDGF contained in BMAC was only minimally changed by the corticotomies. The corticotomies in all pigs healed uneventfully, showing complete obliteration of the corticotomy gaps on CT images. Comparison between the two sides showed that the corticotomy side had thicker and denser cortical bone and more abundant osteogenic cell differentiation than the control side. These findings suggest that the quantity and proliferative/osteogenic differentiation capacity of MSCs contained in local BMAC can be enhanced by minor corticotomies, and spontaneous healing of the corticotomy can be completed within 6 weeks of the operation.Although human brain represents only 2% of the body mass, it uses around 20 % of the organism energy. Due to the brain’s limited energy storage, the oxygen and glucose necessary to support brain functions depends on the correct blood supply. The main components of the arteries are smooth muscle cells, which are considered the main regulators of vascular tone and blood flow distribution. The information currently available on the functioning of the cerebral arteries and their cell constituents is extremely scarce. Thus, the aim of this work was to develop an in vitro model of smooth muscle cells derived from rat middle cerebral artery. Explants were collected from rat middle cerebral artery and adhered to collagen-coated culture dishes. Immunocytochemical analysis showed that the cells present in the culture expressed α-actin, a protein characteristic of the contractile phenotype of these cells. In addition, these cells did not express the endothelial marker, vWF. To evaluate the functionality of these cells the response to contractile agents, serotonin and noradrenaline, and to relaxing agent, sodium nitroprusside was determine by Planar Cell Surface Area analysis. Together the data obtained show that the cell culture obtained through the procedure described resulted in cells presenting the markers characteristic of smooth muscle cells and maintaining the usual contractile response, indicating that the cells obtained through this may be used as a model for characterization and study of functional behavior of the middle cerebral artery, as well as interaction studies between vascular and neuronal system.Male infertility is a global problem in modern society of which capacitating defects are a major cause. Previous studies have demonstrated that Ca2+ ionophore A23187 can make mouse sperm capable of fertilizing in vitro, which may aid in clinical treatment of capacitating defects. However, the detailed role and mechanism of Ca2+ in the capacitating process are still unclear especially how A23187 quickly renders sperm immotile and inhibits cAMP/PKA-mediated phosphorylation. We report that A23187 induces a Ca2+ flux in the mitochondria enriched sperm tail and excess Ca2+ inhibits key metabolic enzymes involved in acetyl-CoA biosynthesis, TCA cycle and electron transport chain pathways resulting in reduced ATP and overall energy production, however this flux does not destroy the structure of the sperm tail. this website Due to the decrease in ATP production, which is the main phosphate group donator and the power of sperm, the sperm is rendered immobile and PKA-mediated phosphorylation is inhibited. Our study proposed a possible mechanism through which A23187 reduces sperm motility and PKA-mediated phosphorylation from ATP generation, thus providing basic data for exploring the functional roles of Ca2+ in sperm in the future.In this work, we present a straightforward method to attach a globular dendrimer at the reducing end of cellulose nanocrystals obtained from tunicates (t-CNC). We investigated the first four generations of poly(amidoamine) dendrimers (PAMAMs G0 to G3) to obtain hybrid t-CNCs. The aggregation behavior of hybrid t-CNCs was studied by dynamic light scattering (DLS) and scanning transmission electron microscopy (STEM); and interactions of these asymmetric nanoparticles with gold surface were elucidated using quartz crystal microbalance with dissipation (QCM-D) and surface plasmon resonance (SPR). Hybrid t-CNCs formed hairy layers onto gold surface combining the properties of rigid rod-like nanocrystals with globular and flexible PAMAM dendrimers. Moreover, the presence of amino groups provided pH-responsive properties to hybrid t-CNCs, and QCM-D results revealed reversible swelling/deswelling behavior. Thereby, we achieved to synthesize tree-shaped functional bio-based materials that adsorbed on gold and formed pH-responsive hairy surfaces.Regeneration of neurites network constitutes a neurotrophic and therapeutic strategy for Parkinson’s disease (PD). Increasing evidence is supporting the potential application of natural polysaccharides in prevention or treatment of PD. In this study, an acidic heteropolysaccharide LFP-1 was isolated from Lycii fructus, and purified by ion-exchange and gel filtration chromatography. Structural features of LFP-1 were analyzed with molecular weight (MW) distribution, monosaccharide composition, methylation and nuclear magnetic resonance (NMR) spectra. LFP-1 was a complicated structured polysaccharide with an average MW of 1.78 × 104 Da and composed of highly branched arabinogalactans, homogalacturonan and rhamnogalacturonan moieties. LFP-1 promoted neuronal differentiation and neurite outgrowth in vitro in PC12 cell models. Furthermore, LFP-1 had a significantly protective effect against 1-methyl-4-phenylpyridiniumion (MPP+)-induced neurotoxicity in PD model PC12 cells. These observations unambiguously indicated the neurotrophic and neuroprotective activities of LFP-1, which may be developed for prevention or treatment of neurodegeneration in PD.Over the past few decades, natural gums are extensively investigated by the researchers due to their beneficial physicochemical properties. Among them, the polysaccharide exudates obtained from the stem of the plant Moringa oleifera, known as moringa gum, is investigated widely in the food, pharmaceutical, and other areas. The moringa gum is used in the form of dried powder as a pharmaceutical excipient in various formulations. It is also derivatized either by grafting or by other chemical modifications for enhancing its properties. The research on moringa gum and modified moringa gum has diversified in numerous biomedical fields. However, summarization of these progress are not available in the literature. This article gives an overview of the collection, purification, structural elucidation, and modification of moringa gum. Moreover, the present review furnishes complete information on the various aspects of moringa gum and its applications in various industrial and biomedical fields.