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Calcareous macroalgae are of particular ecological importance as primary producers, carbonate sediment builders, and habitat providers in coral reef ecosystems. Ocean warming is a major threat to calcareous algae, but it remains unclear exactly how these algae will respond to it. In this study, the potential physiological impacts of ocean warming on the calcareous alga Amphiroa fragilissima were evaluated in laboratory experiments. Increasing temperature from 26 to 28°C had positive effects on algal growth rate and chlorophyll a content, but these parameters decreased significantly at 32°C, which is 5°C above the annual mean temperature in the study region. Algal bleaching occurred at 34°C. There were no significant differences in CaCO3 content of thalli among different temperatures; however, calcification rate was inhibited significantly at 32 and 34°C. Transcriptome analyses using the Illumina RNA-seq platform showed that differentially expressed genes were annotated mainly in the categories of steroid biosynthesis, gap junction, ribosome, and mTOR signaling pathway. The expression levels of PsbA and PsbP were suppressed at 32°C, implying that inactivation of photosystem II could be a main reason for the decreased photosynthetic rate. Down-regulation of the genes encoding carbonic anhydrase and nitrate reductase was observed at 32°C, which could inhibit growth rate. Additionally, several genes that might be related to calcification were identified, including CAMK, CDPK, and CAM and genes encoding alpha-catenin and carbonic anhydrase. This study contributes to our understanding of the effects of temperature on algal calcification and provides a theoretical basis to protect ecological diversity of coral reef ecosystems.De-differentiated chondrosarcoma (DDCS) is an extremely aggressive tumor of the bone characterized by a high-grade, non-chondroid sarcoma adjacent to a low- or intermediate-grade chondrosarcoma. Adequate tumor sampling demonstrating the biphasic features is necessary to make an accurate diagnosis. The diagnosis may be challenging as histopathology may mimic other neoplasms. We present a case of a 76-year-old woman with a history of breast cancer who presented with a pathologic non-displaced fracture. A bone biopsy demonstrated a high-grade neoplasm composed of pleomorphic spindled and epithelioid cells with focal expression of AE1/3 and GATA3, most likely consistent with metastatic breast carcinoma. After a difficult clinical course, the tumor was resected demonstrating a similar morphology to her prior biopsy, as well as an area of a low-grade cartilaginous neoplasm consistent with chondrosarcoma. The biphasic tumor alongside a low-grade chondrosarcoma allowed for a diagnosis of DDCS. Several days after her procedure, the patient developed violaceous nodules overlying and surrounding the surgical site. Skin biopsy demonstrated a malignant epithelioid neoplasm with identical histomorphologic features identical to her prior bone resection. Given the location of the skin lesions directly within the surgical site right after resection, the clinical-pathological picture was that of sarcomatosis cutis by iatrogenic cutaneous implantation.Adolescents’ involvement in cyberbullying has been a growing public health concern for some time. Cybervictimization and cyberaggression are two phenomena that previous research has often shown to be associated. However, longitudinal research into these associations and also into potential risk factors for these phenomena is less common. Anger rumination is a proven risk factor for aggressive behavior, but the relationship between anger rumination and victimization is not clear. The present longitudinal study investigated the associations between cybervictimization, anger rumination and cyberbullying in a sample of 3017 adolescents (MW1  = 13.15; SD = 1.09; 49% girls) from 7th to 9th grade. The European Cyberbullying Intervention Project Questionnaire and the Anger Rumination Scale were administered in four waves with 6 months intervals over a total period of 18 months. The associations between the variables were analyzed with a cross-lagged model. We found that cybervictimization predicted anger rumination and cyberaggression; anger rumination was associated with later increases in both cybervictimization and cyberaggression but involvement in cyberaggression predicted neither subsequent involvement in cybervictimization, nor in anger rumination. In addition, cybervictimization was found to mediate the association between anger rumination and cyberaggression. This study expands the understanding of the factors associated with cybervictimization and cyberaggression, and its results indicate that intervention programs should focus on boosting self-control to decrease impulsive behavior and protocols to prevent and intervene in cyberbullying.Colorectal cancer (CRC) is one of the commonest human cancers and the fourth primary cause of cancer-related death. Zolinza Previous studies have reported that miR-4429 develops anticancer function in follicular thyroid carcinoma and non-small cell lung cancer. However, whether miR-4429 is implicated in the CRC progression remains to be clarified. The aim of our current study was to explore the potential role of miR-4429 in CRC. According to the result of quantitative real-time polymerase chain reaction analysis, miR-4429 was expressed at a low level in CRC cells. Gain-of-function assays showed that the upregulation of miR-4429 inhibited cell proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT) process in CRC, whereas miR-4429 inhibition led to the opposite results. It was uncovered from mechanism experiments that miR-4429 targeted forkhead box M1 (FOXM1) and therefore regulating SMAD family member 3 (SMAD3) expression. Rescue experiments elucidated that miR-4429 influenced cell proliferation, migration, invasion, and EMT process in CRC by targeting FOXM1 to inactivate SMAD3. In conclusion, our study revealed that miR-4429 targeted FOXM1 to decrease SMAD3 expression and thus impeding cell proliferation, migration, invasion, and EMT process of CRC cells.