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The new reference, ARS-UCD1.2, successfully assembled germline immune gene clusters and improved overall continuity (i.e., reduction of gaps and inversions) by over 250-fold. This reference properly places nearly all of the legacy genetic markers used for over a decade in the industry. In this review, we discuss the improvements made to the cattle reference; remaining issues present in the assembly; tools developed to support genome-based studies in beef and dairy cattle; and the emergence of newer genome assembly methods that are producing even higher-quality assemblies for other breeds of cattle at a fraction of the cost. The new frontier for cattle genomics research will likely include a transition from the individual Hereford reference genome, to a “pan-genome” reference, representing all the DNA segments existing in commonly used cattle breeds, bringing the cattle reference into line with the current direction of human genome research. Finding a rapid and simple method of serum IgG determination in lambs is essential for monitoring failure of passive transfer of immunity. The aim of this study was to assess the ability of capillary electrophoresis (CE), an instrument mainly used in blood serum protein analysis, to estimate IgG content in serum of newborn lambs through determination of only total Ig percentage by comparing the results with those obtained with radial immunodiffusion (RID), the reference method for serum IgG quantification. Serum samples were collected at 24 h after birth from 40 Sarda lambs. The IgG concentration measured by RID and serum total Ig concentration measured by CE were (mean ± standard deviation) 29.8 ± 16.1 g/L and 37.8 ± 15.0%, respectively. Data provided by RID and CE analysis showed a polynomial trend (RID = 0.02CE2 – 0.04CE + 4.13; coefficient of determination, R2 = 0.96), displaying a strong relationship between these 2 methods. Applying the polynomial equation, the IgG values were predicted. Predicted IgG values were highly correlated (r = 0.98) and related (R2 = 0.96) to IgG values obtained by RID assay. These data were subjected to Bland-Altman analysis, revealing a high level of agreement between CE and RID methods with a bias that was not different from 0 (-0.04 g/L) and agreement limits of -6.38 g/L (low) and +6.30 g/L (high). In addition, the linear regression analysis between differences (dependent variable) and average of IgG concentration by CE and RID (independent variable) did not show proportional bias (R2 = 0.01). In conclusion, CE is a reliable instrument for a lamb health monitoring program, where Bland-Altman analysis also confirmed that the CE method can be a suitable alternative to RID. Dynamic low-amplitude oscillatory rheology was used to study the gelation properties of skim milk gels made at 37°C, using glucono-δ-lactone alone (acid gels) or a combination of glucono-δ-lactone and porcine pepsin (“combination gels”). The protein contents of the skim milks increased in the order goat milk less then cattle milk less then buffalo milk less then sheep milk less then deer milk, whereas the average casein micelle diameters increased in the order cattle milk less then buffalo milk less then goat milk less then sheep milk ≃ deer milk. The gelation pH (4.55-4.73) of all milks were close to the isoelectric pH (4.6) of casein, except for buffalo milk, which had a significantly higher gelation pH (5.72). The storage moduli (G’) of the acid gels increased with time in the milks of all species except for buffalo milk, for which a double peak in G’ was observed. The final storage moduli after 6 h (G’final) increased in the order goat milk less then cattle milk less then sheep milk less then deer milk less then buffalo milk. In general, for the combination gels, the G’final values and the gelation pH increased to variable extents, except for goat milk. Confocal scanning laser microscopy showed that goat milk and cattle milk formed gels with more open protein networks compared with the dense clustered protein networks of the milks with high protein content (buffalo, sheep, and deer milks). This study indicates that milks from different species respond differently under the action of an acid precursor and pepsin. These results can be used to provide a better understanding of curd making and the digestion properties of noncattle milks. The world has been warming as greenhouse gases accumulate. Worldwide from 1880 to 2012, the average surface temperature has increased by about 0.85°C and by 0.12°C per decade since 1951. The world’s cattle population is a contributor to atmospheric methane, a potent greenhouse gas, in addition to suffering from high temperatures combined with humidity. This makes research into reducing the global footprint of dairy cows of importance on a long-term horizon, while improving tolerance to heat could alleviate the effects of rising temperatures. In December 2017, genomic estimated breeding values for heat tolerance in dairy cattle were released for the first time in Australia. Currently, heat tolerance is not included in the Balanced Performance Index (Australia’s national selection index), and the correlation between heat tolerance breeding values and Balanced Performance Index is -0.20, so over time, heat tolerance has worsened due to lack of selection pressure. However, in contrast, sizable reductions in greenhouse gas emissions have been achieved as a favorable response to selecting for increased productivity, longevity, and efficiency, with opportunities for even greater gains through selecting for cow emissions directly. Internationally considerable research effort has been made to develop breeding values focused on reducing methane emissions using individual cow phenotypes. This requires (1) definition of breeding objectives and selection criteria and (2) assembling a sufficiently large data set for genomic prediction. Selecting for heat tolerance and reduced emissions directly may improve resilience to changing environments while reducing environmental impact. read more OBJECTIVE The aim of this study is to describe the accordion myringoplasty technique as a novel method used in the perforation of the eardrum. MATERIALS AND METHODS The study included thirty patients operated by utilizing accordion myringoplasty technique. RESULTS We achieved complete closure of the eardrum perforations with the accordion myringoplasty technique in all patients. CONCLUSION Accordion myringoplasty technique was inspired by conventional fascial and cartilage myringoplasty techniques to protect hearing while increasing surgical success.