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Currently, FuncPEP includes a comprehensive annotation of 112 functional ncPEPs and specific details regarding the ncRNA transcripts that encode these peptides. We believe that FuncPEP will serve as a platform for further deciphering the biologic significance and medical use of ncPEPs.One of the promising directions in personalized medicine is the use of three-dimensional (3D) tumor models such as spheroids and organoids. Spheroids and organoids are three-dimensional cultures of tumor cells that can be obtained from patient tissue and, using high-throughput personalized medicine methods, provide a suitable therapy for that patient. selleck chemicals llc These 3D models can be obtained from most types of tumors, which provides opportunities for the creation of biobanks with appropriate patient materials that can be used to screen drugs and facilitate the development of therapeutic agents. It should be noted that the use of spheroids and organoids would expand the understanding of tumor biology and its microenvironment, help develop new in vitro platforms for drug testing and create new therapeutic strategies. In this review, we discuss 3D tumor spheroid and organoid models, their advantages and disadvantages, and evaluate their promising use in personalized medicine.Recently, three-dimensional (3D) warp interlock fabric has been involved in composite reinforcement and soft ballistic material due to its great moldability, improved impact energy-absorbing capacity, and good intra-ply resistance to delamination behaviors. However, understanding the effects of different parameters of the fabric on its mechanical behavior is necessary before the final application. The fabric architecture and its internal yarn composition are among the common influencing parameters. The current research aims to explore the effects of the warp yarn interchange ratio in the 3D warp interlock para-aramid architecture on its mechanical behavior. Thus, four 3D warp interlock variants with different warp (binding and stuffer) yarn ratios but similar architecture and structural characteristics were engineered and manufactured. Tensile and flexural rigidity mechanical tests were carried out at macro- and meso-scale according to standard EN ISO 13 934-1 and nonwoven bending length (WSP 90.5(05)), respectively. Based on the results, the warp yarn interchange ratio in the structure revealed strong influences on the tensile properties of the fabric at both the yarn and final fabric stages. Moreover, the bending stiffness of the different structures showed significant variation in both the warp and weft directions. Thus, the interchange rations of stuffer and binding warp yarn inside the 3D warp interlock fabric were found to be very key in optimizing the mechanical performance of the fabric for final applications.The third-stage larvae (L3) of Anisakis simplex are the most important source of hidden allergens in seafood products. However, there exist no commercial methods for detecting Anisakis proteins in food. Furthermore, only a few methods have been validated for the detection of A. simplex in thermally processed food. The aims of our study are (i) the development and validation of high-sensitivity chemiluminescent (CL) immunoassays for the detection of A. simplex proteins in processed seafood, (ii) and A. simplex antigen detection in common seafood products from Polish markets. We developed and validated CL sandwich ELISA (S-ELISA) and CL competitive ELISA (C-ELISA) methods for A. simplex proteins detection in food, with respective detection limits of 0.5 and 5 ng/mL. The usefulness of the assays for detecting A. simplex proteins in highly processed food was evaluated by examination of autoclaved canned fish spiked with A. simplex larvae (1-8 larvae/200 g). Commercial real-time PCR was unable to detect A. simplex in autoclaved samples at all levels of enrichment with Anisakis larvae. CL-S-ELISA was used to test various types of seafood products from Polish markets. Among all tested products (n = 259), 28% were positive. A. simplex antigens were found mostly (n = 39) in smoked fish products mackerel, herring, cod, and hake. Other positive samples were found in marinated herrings, canned cod livers, canned mackerels, and surimi sticks. In tuna, Atlantic argentine, anchovy, sardine, sprat, and squid products, A. simplex antigens were not detected. This study provides novel effective tools for the detection of A. simplex proteins in processed food and highlights the potential allergic hazards for Anisakis-sensitized Polish consumers of seafood.High thermal conductivity insulating dielectrics with good electrical properties have received widespread attention due to the continuous development of power systems and power electronic technologies. In this paper, the effects of differently structured nano alumina fillers on the thermal conductivity and insulating properties of polymer-based composites were studied. It was found that all three types of Al2O3 nano-fillers enhanced the thermal conductivity of the composites, and the thermal conductivity increased more dramatically with increasing filler particle size. It is worth noting that Al2O3 nanowires (NWs) exhibited the most significant improvement in thermal conductivity. The volume resistivity of the composites first increased and then decreased with increasing mass fraction of fillers, and Al2O3 nanoplates (NPLs) showed the most significant improvement in the insulation performance of the composites. The dielectric constants of the composites increased with increasing mass fraction of fillers, while the dielectric losses first decreased and then increased with the same trend, yet the mass fractions of fillers for the three materials were different when the dielectric loss reached a minimum. In addition, all three types of filler increased the AC breakdown strength of the composites, but Al2O3-NPLs showed the most significant improvement on the breakdown performance of the composites.Seizures are common in patients with high-grade gliomas (30-60%) and approximately 15-30% of glioblastoma (GB) patients develop drug-resistant epilepsy. Reliable animal models are needed to develop adequate treatments for glioma-related epilepsy. Therefore, fifteen rats were inoculated with F98 GB cells (GB group) and four rats with vehicle only (control group) in the right entorhinal cortex. MRI was performed to visualize tumor presence. A subset of seven GB and two control rats were implanted with recording electrodes to determine the occurrence of epileptic seizures with video-EEG recording over multiple days. In a subset of rats, tumor size and expression of tumor markers were investigated with histology or mRNA in situ hybridization. Tumors were visible on MRI six days post-inoculation. Time-dependent changes in tumor morphology and size were visible on MRI. Epileptic seizures were detected in all GB rats monitored with video-EEG. Twenty-one days after inoculation, rats were euthanized based on signs of discomfort and pain.