Activity

The present investigationwas carried out to determine the effect of germination on pasting, rheological, morphological properties of Kodo millet flour and in-vitroantioxidant characteristics of its phenolic and γ-amino butyric acid extracts. Rheological analysis depicted complex flour viscosity decreased with an improvement in shear intensity, symbolizing the shear-thinning action of flour upon germination. The frequency and temperature sweep demonstrated a decrease in visco-elasticity as a result of germination wherein, SEM revealed destruction in the continuous composite structure of starch which got entangled in dense protein matrix following germination. The in-vitroantioxidant activities such as total antioxidant capacity, DPPH*, FRAP, metal chelating ability and hydrogen peroxide scavenging activities of both the extracts increased significantly. There was a decrease in pasting properties and gelatinization behaviour whereas, visco-elastic solid behaviour changed to visco-elastic fluid. This research explores the potential of germinated Kodo millet flour for valuable bioactive compounds extraction and its utilization in product development.A new, green, and simple liquid-phase microextraction method namely sieve conducted two syringe-based pressurized liquid-phase microextraction methods was combined with flame atomic absorption spectrometry for the preconcentration and determination of cobalt. For this aim, a novel automated syringe mixing system was developed to be used in the developed extraction procedure. Two syringes were connected to each other by an apparatus having six holes to produce efficient dispersion of the extractant. The pressure created between the two syringes by the forward and backward movements of the syringe plungers created an efficient dispersion of the extractant. In the present study, ligand as complexing agent was synthesized in our laboratory. Limits of detection and quantification were determined to be 1.8 and 6.0 μg L-1, respectively. A 33.7-fold enhancement in detection power was obtained with the developed method. Method was effectively applied for the determination of cobalt in quince samples.Antibiotics are widely used in the prevention and treatment of infectious diseases in animals due to its bactericidal or bacteriostatic action. Residual antibiotics and their metabolites pose great threats to human and animal health, such as potential carcinogenic and mutagenic effects, and bacterial resistances. Therefore, it is necessary and urgent to accurately monitor trace amounts of antibiotics in food samples. Up to now, many analytical methods have been reported for the determination of antibiotics. Biosensors with the advantages of high sensitivity, rapid response, easy miniaturization, and low price have been widely applied to the detection of antibiotics residues in past decades. This review offered an in-depth evaluation of recognition elements for antibiotic residues in diverse food matrices. In addition, it presented a systematical and critical review on signal amplification via various materials, focusing on recently developed nanomaterials. Finally, the review provided an outlook on the future concepts to help upgrade the sensing techniques for antibiotics in food.A magnetic mesoporous molecularly imprinted polymers was synthesized on the surface of magnetic nanoparticles silanized with 3-(trimethoxysilyl) propyl methacrylate to introduce reactive methacrylate groups. Subsequently, methacrylic acid monomers were grafted onto the surface of this adsorbent functionalized via polymerization by precipitation. Magnetic mesoporous molecularly imprinted polymer was properly characterized by different techniques and applied as adsorbent in magnetic solid phase extraction for selective determination of two organophosphorus pesticides, azamethiphos and chlorpyrifos, in mineral water and grape samples. After sample preparation optimization, recoveries of 99.56% and 98.86% were obtained for azamethiphos and chlorpyrifos, respectively. The magnetic solid phase extraction coupled to HPLC-UV presented limit of quantification of 5 ng mL-1, linearity ranged of 5 to 1000 ng mL-1, in addition to adequate accuracy, precision and robustness. The pesticides showed stability in the matrix and were satisfactorily quantified in real mineral water and grape samples.The dissipation and residue levels of emamectin benzoate emulsifiable concentrate (EC) and microemulsion (ME) formulations in tender cowpeas and old cowpeas were investigated under field conditions. The decline curves of emamectin benzoate residues in cowpea corresponded to first-order kinetics. The dissipation rate of emamectin benzoate in tender cowpeas was faster than that in old cowpeas. The half-lives of the EC were 1.34-1.39 d and 1.74-2.31 d in tender cowpea and old cowpea, respectively. For the ME, the half-lives were 1.39-1.51 d and 2.08-2.67 d, respectively. learn more The risk of adult intake of emamectin benzoate from cowpea is within the acceptable limits of the human body. Compared to tender cowpeas, the risk of eating old cowpeas is higher. Emamectin benzoate (EC) is recommended for cowpeas when the intention is to harvest tender cowpeas, while both formulations are acceptable for cowpeas when the intention is to harvest old cowpeas.Seaweeds are increasingly used in European cuisine. Until the recent use of molecular techniques, species identification was solely based on morphology which cannot easily discriminate morphologically simple but phenotypically plastic taxa such as the green algal genus Ulva. For example, current taxonomic protocol effectively reassigned the previously known European ‘Ulva lactuca L.’ under the name Ulva fenestrata Postels & Ruprecht. Also, the presumptive Ulva lactuca approved by the Institute for Reference Materials and Measurements (IRMM, Joint Research Center, European Commission) as Certified Reference Material (CRM) for analytical quality assurance was genetically identified as U. rigida C.Agardh. It is very likely that different Ulva species under various names have been consumed as food not only in Europe, but also worldwide. In this regard, when chemical composition and nutritional quality of different seaweed species meet a set of food standard criteria, and food safety hazards are mitigated, they should be endorsed for consumption.