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The ongoing coronavirus disease 19 (COVID-19) pandemic, caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), poses a threat to human health. Despite this, many affected countries are now in the process of gradual lifting of COVID-19 restrictions that were initially implemented in response to the pandemic. The success of the so-called “exit strategy” requires continued surveillance of virus circulation in the community and evaluation of the prevalence of protective immunity among population. Serology tests are valuable tools for these purposes. Herein, SARS-CoV-2 full-length spike (S) recombinant protein was utilized to develop and optimize an indirect enzyme-linked immunoassay (ELISA) that enables a reliable detection of virus-specific IgG antibody in human sera. Importantly, the performance of this assay was evaluated utilizing micro-neutralization (MN) assay as a reference test. Our developed ELISA offers 100% sensitivity, 98.4% specificity, 98.8% agreement, and high overall accuracy. Moreover, the optical density (OD) values of positive samples significantly correlated with their MN titers. The assay specifically detects human IgG antibodies directed against SARS-CoV-2, but not those to Middle East respiratory syndrome coronavirus (MERS-CoV) or human coronavirus HKU1 (HCoV-HKU1). The availability of this in-house ELISA protocol would be valuable for various diagnostic and epidemiological applications.The purpose of this study was to synthesize and characterize novel biocompatible topical polymeric film and hydrogel systems that have the potential to deliver the antibacterial agent thymoquinone (TQ) directly to the skin target site to manage the local wound infection and thereby wound healing. The polyvinyl pyrrolidone (PVP) matrix-type films containing TQ were prepared by the solvent casting method. In vitro skin permeation studies on human cadaver skin produced a mean flux of 2.3 µg TQ/cm2/h. Human keratinocyte monolayers subjected to a scratch wound (an in vitro wound healing assay) showed 85% wound closure at day 6 in the TQ group (100 ng/mL TQ) as compared to 50% in the vehicle control group (p = 0.0001). In a zone-of-inhibition (ZOI) assay, TQ-containing films and hydrogels completely wiped out Staphylococcus aureus in 10 cm diameter Tryptic Soy Agar plates while 500 µg/mL gentamicin containing filters gave 10 mm of ZOI. In an ex vivo model, TQ-containing films eradicated bacterial colonization on human cadaver skin. Furthermore, in a full-thickness wound infection model in mice, TQ-containing films showed significant activity in controlling Staphylococcus aureus infection, thereby disinfecting the skin wound. Tauroursodeoxycholic In summary, TQ-containing PVP films and hydrogels developed in this study have the potential to treat and manage wound infections.Photonic true time delay beam steering on the transmitter side of terahertz time-domain spectroscopy (THz TDS) systems requires many wideband variable optical delay elements and an array of coherently driven emitters operating over a huge bandwidth. We propose driving the THz TDS system with a monolithic mode-locked laser diode (MLLD). This allows us to use integrated optical ring resonators (ORRs) whose periodic group delay spectra are aligned with the spectrum of the MLLD as variable optical delay elements. We show by simulation that a tuning range equal to one round-trip time of the MLLD is sufficient for beam steering to any elevation angle and that the loss introduced by the ORR is less than 0.1 dB. We find that the free spectral ranges (FSRs) of the ORR and the MLLD need to be matched to 0.01 % so that the pulse is not significantly broadened by third-order dispersion. Furthermore, the MLLD needs to be frequency-stabilized to about 100 MHz to prevent significant phase errors in the terahertz signal. We compare different element distributions for the array and show that a distribution according to a Golomb ruler offers both reasonable directivity and no grating lobes from 50 GHz to 1 THz.With the improvement of semiconductor technology, the performance of CMOS Image Sensor has been greatly improved, reaching the same level as that of CCD in dark current, linearity and readout noise. However, due to the production process, CMOS has higher fix pattern noise than CCD at present. Therefore, the removal of CMOS fixed pattern noise has become the research content of many scholars. For current fixed pattern noise (FPN) removal methods, the most effective one is based on optimization. Therefore, the optimization method has become the focus of many scholars. However, most optimization models only consider the image itself, and rarely consider the structural characteristics of FPN. The proposed sparse unidirectional hybrid total variation (SUTV) algorithm takes into account both the sparse structure of column fix pattern noise (CFPN) and the random properties of pixel fix pattern noise (PFPN), and uses adaptive adjustment strategies for some parameters. From the experimental values of PSNR and SSM as well as the rate of change, the SUTV model meets the design expectations with effective noise reduction and robustness.To study the regulation of sulfate metabolism in barley (Hordeum vulgare), seedlings were exposed to atmospheric hydrogen sulfide (H2S) in the presence and absence of a sulfate supply. Sulfate deprivation reduced shoot and root biomass production by 60% and 70%, respectively, and it affected the plant’s mineral nutrient composition. It resulted in a 5.7- and 2.9-fold increased shoot and root molybdenum content, respectively, and a decreased content of several other mineral nutrients. Particularly, it decreased shoot and root total sulfur contents by 60% and 70%, respectively. These decreases could be ascribed to decreased sulfate contents. Sulfate deficiency was additionally characterized by significantly lowered cysteine, glutathione and soluble protein levels, enhanced dry matter, nitrate and free amino acid contents, an increased APS reductase (APR) activity and an increased expression and activity of the root sulfate uptake transporters. When sulfate-deprived barley was exposed to 0.6 µL L-1 atmospheric H2S, the decrease in biomass production and the development of other sulfur deficiency symptoms were alleviated.