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Concisely, the GSLM with coefficients of determination (R2) 99.957% and root mean squared error (RMSE) of 2.121% outperformed the SVM-FFA with R2 99.59%, RMSE 3.27%, ANN with R2 99.56%, RMSE 3.3%, ANFIS with R2 98.9%, RMSE 4.3%, GP with R2 99.89%, RMSE 3.47%, GEP with R2 94.75%, RMSE 4.15% for forecasting weekly time series. In forecasting monthly time series, the GLSM method with R2 99.517% and RMSE 6.91% also outperformed GEP R2 91.95%, RMSE 15.3%, ANFIS R2 92.85%, RMSE 47.55% models. Consequently, GSLM proved that by applying proper comprehensible linear techniques promising results can be obtained rather than using sophisticated AI methods. BACKGROUND This study evaluated vulnerable subpopulation on mortality, emergency room visits (ERVs) and outpatient visits associated with ambient daily temperature from 2000 to 2014 using vital statistics and insurance claims of Taiwan. METHODS We used the distributed lag non-linear model to assess circulatory disease-specific deaths, ERVs, and outpatient visits by mean temperature after controlling particulate matter (PM10) and other covariates. Lag effect of temperature changes on health risks accumulated for 0-10 days associated with low temperature and for 0-5 days for high temperature were evaluated. Cause-specific pooled relative risk (RR) and 95% confidence intervals (CI) were estimated for the whole population of Taiwan using random-effects meta-analysis. RESULTS We used reference temperatures of 60th percentiles for mortality from circulatory diseases, 99th percentile for ERVs of circulatory diseases, 2nd percentile for ERVs of heart diseases and ischemic heart disease, 53th percentile for ERVs of cerebrovascular disease, and 12-16th percentiles for outpatient visits of circulatory diseases. The lag effects peaked at lag 4-5 day for low temperature exposure and at lag 0 for high temperature exposure. Pooled cold related health risk was the highest for mortality from and ERV of circulatory diseases with RR of 1.41 (95% CI 1.34, 1.49) and 1.41 (95% CI 1.35, 1.48), respectively, as daily mean temperatures was at 1st percentile (12.8 °C). Heat related health risk was significant for mortality from heart diseases [RR = 1.12 (95% CI 1.07, 1.18)] and ischemic heart diseases [RR = 1.13 (95% CI 1.06, 1.20)] as daily mean temperatures was at 99th percentile (29.9 °C). CONCLUSIONS Health authority should evaluate the effectiveness of adaptive policy, strategy, and actions responding to extreme temperatures to prevent mortality from circulatory diseases. V.There are few studies about biochar application in seasonally frozen soil areas. TLR2-IN-C29 The regulatory mechanism of biochar on the water-soil environment and carbon emissions in seasonally frozen soil areas is unclear, which affects the study of nutrient migration and spring cropping systems under the control of biochar. For this purpose, we monitored the soil temperature (Ts), soil liquid moisture content (Ms) and soil respiration (Rs) rate during the freeze-thaw period under different application amounts of corn stover biochar (0 t∙ha-1, 15 t∙ha-1, 30 t∙ha-1, 45 t∙ha-1 and 60 t∙ha-1). The results showed that biochar can reduce the thermal conductivity of soil, thus improving the thermal insulation effect of frozen thawed soil, and Ts increased by 1.8-5.7 °C. The Ts and Ms were more sensitive to the high biochar application amount than to the low application amount. At the same time, biochar changed the soil aggregate distribution, and Pearson correlation analysis indicated that the soil water retention capacity increased by increasing the macroaggregate content (>0.25 mm), and the Ms increased by 3.7-6.1%. Principal component regression (PCR) analysis showed that biochar can promote soil carbon emission, and Rs of soil treated with biochar was 0.01-0.58 μmol m-2 s-1 higher than that of the control. The Ms and Ts were the most important factors promoting the carbon emissions of freeze-thaw soil under the synergistic effect of biochar and freeze-thaw conditions. However, biochar may promote soil CO2 emissions by affecting the water-soil environment. Considering the soil moisture, seed germination and growth conditions in spring, the suitable biochar application amount was determined to be 44-51 t∙ha-1. This study provides theoretical support for determining reasonable and effective biochar control measures and improving the soil productivity of farmland soil in seasonally frozen soil areas. V.Understanding the extent to which laboratory findings of low pH on marine organisms can be extrapolated to the natural environment is key toward making better projections about the impacts of global change on marine ecosystems. We simultaneously exposed larvae of the sea urchin Arbacia lixula to ocean acidification in laboratory and natural CO2 vents and assessed the arm growth response as a proxy of net calcification. Populations of embryos were simultaneously placed at both control and volcanic CO2 vent sites in Ischia (Italy), with a parallel group maintained in the laboratory in control and low pH treatments corresponding to the mean pH levels of the field sites. As expected, larvae grown at constant low pH (pHT 7.8) in the laboratory exhibited reduced arm growth, but counter to expectations, the larvae that developed at the low pH vent site (pHT 7.33-7.99) had the longest arms. The larvae at the control field site (pHT 7.87-7.99) grew at a similar rate to laboratory controls. Salinity, temperature, oxygen and flow regimes were comparable between control and vent sites; however, chlorophyll a levels and particulate organic carbon were higher at the vent site than at the control field site. This increased food availability may have modulated the effects of low pH, creating an opposite calcification response in the laboratory from that in the field. Divergent responses of the same larval populations developing in laboratory and field environments show the importance of considering larval phenotypic plasticity and the complex interactions among decreased pH, food availability and larval responses. Among the emerging contaminants today, pharmaceuticals are some of the most demanding chemical compounds when it comes to understanding their transport within aquifers. The transport of pharmaceuticals in an unsaturated zone is influenced by many factors, including compound sorption and degradation, which is essential in assessing contaminant migration in soil and groundwater. Coarse-gravel aquifers are particularly important for drinking water sources and industrial water supply. Globally, little data on the transport characteristics of coarse-gravel unsaturated zones is available. However, such data is crucial to understanding the transport of pollutants and to implementing the appropriate management strategies to protect the aquifers. In this article, we present tracer experiments employed to determine pharmaceutical transport parameters in the coarse-gravel unsaturated zone. The tracer experiment was performed as a multi-tracer exercise, where deuterated water was infiltrated as a conservative tracer to define the characteristics of the unsaturated zone, together with pharmaceuticals (propyphenazone, caffeine and carbamazepine) as reactive tracers.