Activity

Among the predictor variables investigated in our study, only balance impairments and depressive symptoms explained the variance in physical activity levels in individuals with Parkinson’s disease.

Oxidation contributes to secondary brain injury after spontaneous intracerebral haemorrhage (SIH). One study found lower levels of total antioxidant capacity (TAC) in the blood in patients with SIH than in healthy subjects. However, there are no data on blood TAC levels and survival in patients with SIH. Therefore, the objective of our study was to determine if an association exists between serum TAC levels and mortality in patients with SIH.

We included patients with severe supratentorial SIH. We considered severe when Glasgow Coma Scale (GCS) < 9. Patients from 6 Spanish hospitals were included in this observational and prospective study. Serum TAC levels at days 1, 4 and 8 of SIH were determined. Thirty-day mortality was our end-point study.

Non-surviving patients compared with surviving patients showed higher serum TAC levels at day 1 (p < 0.001), 4 (p < 0.001) and 8 (p = 0.001). An area under the curve was found for the prediction of 30-day mortality by serum TAC levels of 0.92 (95% CI = 0.85-96%; p < 0.001). Multiple logistic regression analysis showed an association of serum TAC levels with 30-day mortality (odds ratio = 16.513; 95% CI = 2.548-107.015; p = 0.003) controlling for midline shift, glycemia, early evacuation of SIH, intracerebral haemorrhage (ICH) score, age and volume of SIH.

The new findings of this study are that serum TAC levels are higher in non-surviving than in surviving patients, and that they are associated with mortality and could be used to predict mortality.

The new findings of this study are that serum TAC levels are higher in non-surviving than in surviving patients, and that they are associated with mortality and could be used to predict mortality.Two sets of mononuclear N, O-chelate complexes, 1a [Co(L1)2(H2O)2], 1b [Ni(L1)2] and 1c [Cu(L1)2], and 2a [Co(L2)2(H2O)2], 2b [Ni(L2)2] and 2c [Cu(L2)2], from respective Schiff base ligands, HL1 2-((E)-(6-(trifluoromethoxy)phenylimino)methyl)-5-methylphenol, C15H12F3NO2) and HL2 2-((E)-(4-(trifluoromethoxy)phenylimino)methyl)-4,6-dichlorophenol, C14H8Cl2F3NO2) have been synthesized and well-characterized by analytical and various spectroscopic techniques like mass spectrometry, FT-IR, UV-visible, magnetic susceptibility, ESR and thermogravimetric studies. The geometries are proposed as octahedral for complexes 1a and 2a, whereas square planar for complexes 1b, 1c, and 2b, 2c. DNA binding study was investigated against calf thymus DNA for synthesized complexes comparing with parent ligands and resulted in intercalation mode of binding which was further supported by fluorescence quenching and viscometric titrations. Nuclease efficacy was also investigated against supercoiled pBR322 DNA and a good cleavage pattern was shown by metal complexes. The complexes were found to possess good antimicrobial activity compared to free ligands against bacterial species, Bacillus amyloliquefaciens and Escherichia coli as well as fungal species, Macrophomina phaseolina and Sclerotium rolfsii.The synthesis of 1-benzyl-2-((2-Aminoethyl) amino)-5-oxopyrrolidine-3,4-diyl diacetate (boad), an oxopyrrolidine type ligand; designed to coordinate lanthanides (Eu3+ and Tb3+) to get luminescent material. The target complexes showed good photoluminescence properties, which indicate that this type of compound can be used as sensitizers having luminescence for the green (Tb3+) and red (Eu3+) emission. The obtained results revealed that sensitizer efficiency can be improved by adding ligands like acac (Eu(acac)3, which has also enhanced the luminescence quantum output and period for Eu3+ ions. The ground state geometries were developed by using density functional theory at B3LYP/6-31G** level. check details The charge transfer analysis and electronic properties were performed. The Europium and Terbium complexes formation with boad ligand was explored based on molecular electrostatic potential, MDC-q charges, and frontier molecular orbitals (FMOs) analysis.Cysteine (Cys) is an important endogenous amino acid and plays critical physiological roles in living systems. Herein, an endoplasmic reticulum (ER)-targeting fluorescent probe (FER-Cys) was designed and prepared for imaging of Cys in living cells. The probe FER-Cys consists of a fluorescein framework as the fluorescent platform, acrylate group as the response site for the selective recognition of Cys, and ER-specific p-toluenesulfonamide fragment. After the response of probe FER-Cys to Cys, a turn-on fluorescence signal at 546 nm could be detected obviously. The probe FER-Cys further shows desirable selectivity to Cys. Finally, the probe FER-Cys was proven to selectively detect Cys in live cells and successfully image the changes of Cys level in the cell models of H2O2-induced redox imbalance.In this study, two metal Schiff base complexes (cobalt(II) and manganese(II), were synthesized and characterized by 1H-NMR and FT-IR analyses. The in vitro binding behavior of this complexes with β-Casein (β-CN) was investigated by using biophysical techniques. For evaluation the thermodynamics parameters of interaction between Schiff base complexes and β-CN,the fluorescence data at different temperatures were done. The results showed that the intrinsic fluorescence of the β-CN was quenched by increasing the complexes through the dynamic quenching mechanism. Also, these complexes demonstrated a considrable binding affinity to β-CN and the process is mainly entropy driven. Fluorescence resonance energy transfer was used in order to estimating the distance between donor (β-CN) and acceptor (complex). Results demonstrated that the sequence of tendency of the complexes to β-CN was as follows Mn-Schiff base complex > Co-Schiff base complex. All these experimental results propose that β-CN might act as carrier protein for Co(II) and Mn (II) complexes to deliver it to the target molecules.Label-free characterization of cell subpopulations is a very promising biomedical approach. Nowadays, there are several label-free methods based on different physical properties such as size, density, stiffness, etc. allowing the characterization of biological objects. However, fluorescence properties are the most suitable feature for the label-free study of tissue and cells. Understanding the autofluorescence level peculiarities of normal and pathological / live and dead cells can become a helpful tool for cells’ metabolic activity, viability evaluation, and diagnostics of a number of diseases. In this study, we applied a series of mouse cell lines (RAW 264.7 – macrophages, L929 – fibroblasts, C2C12 – myoblasts, and B16-F10 – melanoma) to compare cell autofluorescence of live and dead cells under 488 nm laser excitation and found the difference between their autofluorescence depending on a cell state and type.