Schwartz

Stem cells are widely explored in regenerative medicine as a source to produce diverse cell types. Despite the wide usage of stem cells like mesenchymal stem cells (MSCs) and induced pluripotent stem cells (iPSCs), there is a lack of robust methods to rapidly discern the phenotypic and functional heterogeneity of stem cells. The organization of…[Read more]

Sample preparation for NMR studies of G-protein coupled receptors faces special requirements Proteins need to be stable for prolonged measurements at elevated temperatures, they should ideally be uniformly labeled with the stable isotopes 13C, 15N, and all carbon-bound protons should be replaced by deuterons. In addition, certain NMR experiments…[Read more]

gic neurons per ganglion, and reduced markers of neurogenesis compared with mice given saline. Colonic LMMP of mice given the TLR4 inhibitor did not have reduced markers of neurogenesis. Colonic LMMP of germ-free mice given TLR2 agonist had increased neuronal numbers compared with control germ-free mice. CONCLUSIONS In the adult mouse colon, TLR2…[Read more]

Observations of microplastic particles (MPs) in the environment and their detection in the stomachs and intestines of aquatic organisms have been observed routinely over the last 50 years. In this review, the ingestion of plastic debris of varying sizes is collated, including data for >800 species representing approximately 87,000 individual…[Read more]

In a genetic mutant model of retinitis pigmentosa, a lead compound, Q525, afforded sustained RET activation and prevented photoreceptor neuron loss in the retina. This work uncovers key components of the dynamic relationships between RET and its GFRa co-receptor and provides RET agonist scaffolds for drug development. Published under license by…[Read more]

The construct of reliability in health professions education serves as a measure of the congruence of interpretations across assessment tools. selleck chemicals When used as an assessment strategy, healthcare simulation serves to elicit specific participant behaviors sought by medical educators. In healthcare simulation, reliability often refers…[Read more]