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Several SARS-CoV-2-related tools/databases were reviewed, and a web-portal named OverCOVID (http//bis.zju.edu.cn/overcovid/) is constructed to provide a detailed interpretation of SARS-CoV-2 basics and share a collection of resources that may contribute to therapeutic advances. These information could improve researchers’ understanding of SARS-CoV-2 and help to accelerate the development of new antiviral treatments.Neoadjuvant immunotherapy involves administering immune checkpoint inhibitors before surgical resection in high-risk resectable disease. This strategy was shown to have a high pathological response rate and prolonged relapse-free survival in randomized trials in melanoma, glioblastoma, and colon cancer with small numbers of patients. In resectable cancers, immune checkpoint inhibitors such as anti-programmed cell death-1 (PD1) and anti-cytotoxic T-lymphocyte-associated protein-4 (CTLA-4) can enhance antitumor immunity by activating antigen-specific T cells found in the primary tumor. These tumor-reactive T cells continue to exert antitumor effects on remaining neoplastic cells after the resection of the primary tumor, potentially preventing relapses from occurring. Based on the scientific rationale and early clinical observations with surrogate survival endpoints, neoadjuvant immunotherapy may provide an effective alternative to other therapeutic strategies such as adjuvant treatment. However, this can be determined only by conducting randomized controlled trials comparing neoadjuvant immunotherapy with the current standard of care for each tumor site. This review discusses the cellular mechanisms that occur during successful neoadjuvant immunotherapy and highlights the clinical data from the available human studies that support the preclinical mechanistic data. Here we also discuss strategies required for successful neoadjuvant immunotherapy, including combination treatment strategies and resistance mechanisms to neoadjuvant treatment.

Eosinophilic heart disease (EHD) is a rare cardiac condition with a wide spectrum of phenotypes. The diagnostic and prognostic value of cardiac magnetic resonance (CMR) in EHD remains unknown.

This was a retrospective analysis of 250 patients with eosinophilia referred for a CMR scan (period 2000-2020). CMR data sets and clinical/laboratory data were collected. Patients were followed up for a mean of 24 months (range 1-224) for the composite endpoint of death, acute coronary syndrome, hospitalization for acute heart failure, malignant ventricular arrhythmias, or the need for implantable cardiac defibrillator/pacemaker. The main objectives were to explore the diagnostic value of CMR in EHD; relationships between cardiac function, late gadolinium enhancement (LGE), and EHD phenotypes; and the prognostic value of fibrosis and oedema by CMR. The prevalence of findings compatible with EHD was 39% (patients with cardiac symptoms 57% vs. screening 20%, P < 0.001). EHD phenotypes included subendocardial LGE (n and independently predicts clinical outcomes in patients with eosinophilia.The aetiology of low birth weight (LBW) is the outcome of complex interactions of numerous physical and environmental factors. Present study aims to identify the factors determining LBW in India as well as estimate the prevalence of LBW. The study used the data of latest National Family Health Survey (NFHS), a nationally representative sample consisting 81,869 ever married Indian women of reproductive age. Data on birth weight of the full-term singleton infants born within three years prior to survey and background characteristics of mothers were obtained from 57,582 mother-infant pairs. Birth weight less than 2500 g was considered as LBW. Descriptive statistics and logistic regression were used for data analysis. Prevalence of LBW was highest in Central region of India (20.73%). Mother’s area of residence, education, economic condition, habit of tobacco chewing appeared as the significant determinants of LBW in infants. Mothers receiving no food supplementation during pregnancy, skipping ANC visits and vaccination, having caesarean or home-based delivery had greater odds of giving birth to LBW children. Female child had greater risk of LBW. The impact of low education reflects in present study in terms of lack of awareness regarding antenatal care, ignorance towards health check-ups and immunization during pregnancy. The study highlighted that low maternal education is an important key determinant to deal with in order to eradicate its major role in determining LBW among the infants.Luminescent nanoclusters (NCs), with their easy preparation, ultrafine size, low toxicity, and excellent photostability have recently emerged as novel electrochemiluminescence (ECL) emitters. However, relatively low quantum yield (QY) in both aqueous and organic media has impeded their application in ECL emitter evolution. In this mini-review, we discuss the recent development of NCs in ECL with particular focus on their optical properties. We first classify the NCs according to composition and structure, and then summarize four aspects that affect QY, including environment effects, construction, valence state effects and aggregation-induced ECL. MYF0137 The ECL mechanisms based on NCs are elucidated as well. Finally, we briefly discuss the potential applications of NCs in tumor markers test, immunoassay and serum test. This review aims to provide a comprehensive summary of the progress of NCs in ECL, which will motivate researchers to develop NC chemistry and explore their future applications in ECL.The use of the specific binding properties of monoclonal antibody fragments such as single-chain variable fragments (ScFv) for the selective delivery of antitumor therapeutics for cancer cells is attractive due to their smaller size, low immunogenicity, and low-cost production. Although covalent strategies for the preparation of such ScFv-based therapeutic conjugates are prevalent, this approach is not straightforward, as it requires prior chemical activation and/or modification of both the ScFv and the therapeutics for the application of robust chemistries. A non-covalent alternative based on ScFv fused to maltose-binding protein (MBP) acting as a binding adapter is proposed for active targeted delivery. MBP-ScFv proves to be a valuable modular platform to synergistically bind maltose-derivatized therapeutic cargos through the MBP, while preserving the targeting competences provided by the ScFv. The methodology has been tested by using a mutated maltose-binding protein (MBP I334W) with an enhanced affinity toward maltose and an ScFv coding sequence toward the human epidermal growth factor receptor 2 (HER2).