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Hvass posted an update 1 year, 3 months ago
This work presents a large-scale three-fold annotated, low-cost microscopy image dataset of potato tubers for plant cell analysis in deep learning (DL) framework which has huge potential in the advancement of plant cell biology research. Indeed, low-cost microscopes coupled with new generation smartphones could open new aspects in DL-based microscopy image analysis, which offers several benefits including portability, easy to use, and maintenance. However, its successful implications demand properly annotated large number of diverse microscopy images, which has not been addressed properly- that confines the advanced image processing based plant cell research. Therefore, in this work, a low-cost microscopy image database of potato tuber cells having total 34,657 number of images, has been generated by Foldscope (costs around 1 USD) coupled with a smartphone. This dataset includes 13,369 unstained and 21,288 stained (safranin-o, toluidine blue-o, and lugol’s iodine) images with three-fold annotation based on weight, section areas, and tissue zones of the tubers. The physical image quality (e.g., contrast, focus, geometrical attributes, etc.) and its applicability in the DL framework (CNN-based multi-class and multi-label classification) have been examined and results are compared with the traditional microscope image set. The results show that the dataset is highly compatible for the DL framework.Increasing the local concentration of microbubbles (MBs) within the blood flow plays a crucial role in several medical applications, but there are few imaging modalities available for volumetric tracking of the aggregated MBs in real time. Here we describe a device integrating acoustic vortex tweezers (AVT) and ultrasound plane-wave imaging to achieve the goal of controlling the spatial distribution of MBs in blood vessels and simultaneously monitoring this process using the same probe. Experiments were conducted using a 5-MHz 2D array ultrasound probe (with three cycles of excitation at an acoustic pressure of 2000 kPa) and 1.2-μm-diameter MBs at a flow rate of 20 mm/s. The AVT waveform was produced by modulating the repetition frequency of the transmitted pulse asymmetrically (4 and 8 kHz at the inflow and outflow ends, respectively). In order to simultaneously capture MBs and perform imaging with the same probe, the asymmetric AVT pulse signal and the ultrasound-imaging pulse signal were arranged in a staggered series, and the imaging was performed using plane-wave pulses at nine angles (-7° to 7°) in compounded plane-wave imaging (volume rate 200 Hz). Microscopy observations showed that freely suspended MBs could indeed be gathered by the asymmetric AVT in the flow field to form an MBs cluster with a spot size of about 4022 μm2, which could resist the flow to remain at a fixed location for about 22 s. After the asymmetric AVT signal and the ultrasound-imaging pulse signal was turned on for 1 s, the ultrasound 3D image showed that the signal intensity of the MB clusters increased by 13.1±2.9 dB relative to the background area. These results show that the proposed strategy can be used to accumulate flowing MBs at a desired location and to simultaneously observe this phenomenon. DDR1-IN-1 manufacturer This tool could be used in the future to improve the outcomes of MB-related treatments for various diseases.Tuberculosis (TB) is still a worldwide problem. We present a case of flexor tenosynovitis due to tuberculosis in the hand and wrist. A 42-year-old man presented to the outpatient clinic with a 2-year history of a slowly growing mass over the volar aspect of the left wrist. His MRI showed multiple rice bodies in the wrist and hand. An open biopsy was performed. Pathology specimens showed granulomatous lesions with central necrosis. The purified protein derivative (PPD) test was positive. In this case, granulomatous lesions with central necrosis, rice bodies, and positive PPD test confirmed the diagnosis of TB in the wrist and hand. There was no other concurrent evidence of TB elsewhere. Antituberculosis chemotherapy was commenced. Tuberculous tenosynovitis of the wrist and hand is very rare. The tuberculous tenosynovitis should be kept in mind as an infectious agent when patients are presenting with atypical clinical. Key words tuberculosis, rice bodies, flexor tenosynovitis, wrist, hand.In patients with defect bone growth and dwarfism, fractures of the weight bearing skeleton are relatively rare due to their reduced mobility. When they do occur, their treatment and potential surgery are complicated. The commonly used therapeutic procedures are not applicable, the available implants are not suitable, if a surgery is necessary. An individual approach and often times also improvisation is needed. It is important to realize that these patients do not suffer from an intellectual disability and are fully aware of their physical impairment, which we must not make worse without an effort for adequate treatment. This case study presents our solution of a supracondylar femoral fracture in a patient with achondroplasia and extreme obesity. A proximal humeral nail was used for distal femoral fracture osteosynthesis by a retrograde approach. To the knowledge of authors, this study reports on the first case of supracondylar femoral fracture surgery in patients with achondroplasia. Key words achondroplasia, dwarfism, supracondylar femoral fracture, extreme obesity.PURPOSE OF THE STUDY The purpose of this study is to reveal the waste of water by medical staff hand scrubbing preoperatively in the operation theatre, draw attention to the water consumption with small precautions. The study compares motion-sensitive sensor tap with an uncensored classic tap, to assess the difference of water consumption during hand scrubbing. MATERIAL AND METHODS The presented study compared two groups; hand scrubbing with sensory tap was enrolled as group A and classic tap with running water was enrolled as group B. Three participants were included in each group. Operation faucets with a motion-sensitive sensor and timekeeper were used in group A. Running water from a tap in 10 seconds was measured with the help of a measuring cup. The water amount after 3 minutes of washing was also measured. The difference between the two obtained data was compared. The obtained data were also multiplied to the global surgical data. RESULTS Measurements were performed with a measuring cup and it was measured that 1,250 ml of water is running from faucets in 10 seconds.