Activity

on behalf of the European Association for Cardio-Thoracic Surgery. All rights reserved.BACKGROUND The ears increase in all dimensions over the years (eg, length, helix diameter, lobe length), but the most obvious change is the elongation or ptosis of the lobe. Its correction should be considered a part of facial rejuvenation. OBJECTIVES The authors presented the surgical procedure they have been utilizing to correct elongated earlobes. METHODS The authors described a simple and effective procedure consisting of the curved resection of the excess tissue of the lobe at its lower end and its reconstruction through the utilization of 2 triangular flaps of skin, taken from the lateral surface of the lobe, rotated backwards, and sutured to the medial surface, leaving a zigzag hidden scar. RESULTS The authors have utilized this surgical procedure over the last 16 years to correct ptosis of the earlobe as well as some of its other alterations due to aging. The results obtained are presented as well as the classification that the authors have utilized to qualify their degree of ptosis. CONCLUSIONS This procedure has yielded excellent results, with imperceptible scars, and is therefore very pleasing to patients and surgeon alike. Level of Evidence 4. © 2020 The Aesthetic Society. Reprints and permission journals.permissions@oup.com.Methionine restriction (MR) extends lifespans in multiple species through mechanisms that include enhanced oxidative stress resistance and inhibition of insulin/insulin-like growth factor I (IGF-I) signaling. Methionine and S-adenosylmethionine (SAM) are the essential precursors of bacterial quorum sensing (QS) molecules, and therefore, MR might also affect bacterial communication to prevent enteric bacterial infection as well as chronic inflammation, which contributes to lifespan prolongation. Here, we discuss the influence of MR on oxidative stress resistance and inhibition of insulin/IGF-I cell signaling and further propose a potential mechanism involving bacterial QS inhibition for lifespan extension. Unraveling the connection between MR and inhibition of QS provides new strategies for combating infectious diseases, resulting in enriched understanding of MR-induced lifespan extension. Copyright © The Author(s) 2020.We determined the potential of the entomopathogenic fungus Metarhizium brunneum Petch (Hypocreales Clavicipitaceae) F52 strain, and of a microsclerotial formulation, for the control of the annual bluegrass weevil, Listronotus maculicollis Kirby, which is a major pest of golf course turf in eastern North America with widespread insecticide resistance. Under laboratory conditions in Petri dishes with moist sand, the microsclerotia (23-46 kg granules/ha) caused high rates of mortality (85-100%) and infection (67-80%) in annual bluegrass weevil adults, but these levels did not occur until after 9 d at constant 26°C and 12-15 d at 14 h at 23°C and 10 h at 17°C. Production of viable conidia was marginally higher at the higher temperature regime (7.3 vs. 5.2 × 109 per gram of granules). Application of microsclerotia did not provide significant control and infection of adults in pots with grass in the greenhouse. In field trials targeting spring generation larvae, microsclerotia application (50-100 kg granules/ha) was ineffective, and coapplication of hydrogel to stabilize soil moisture did not increase larval control. A liquid M. brunneum F52 conidial formulation (4.75-9.5 × 1013 colony forming units/ha) provided up to 51% control. Combinations of M. brunneum F52 with the neonicotinoid insecticide imidacloprid provided additive control with up to 70% control with the conidial formulation. Field efficacy was probably limited by suboptimal temperatures for the fungus, and future tests need to examine whether higher control rates can be achieved in applications targeting the summer generation larvae. © The Author(s) 2020. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. ICI-118551 nmr For permissions, please e-mail journals.permissions@oup.com.ABSTRACT Absent, small, or homeotic discs 2 (Ash2), a histone H3K4 methyltransferase complex, has been implicated in the control of hyphal development and secondary metabolism in many kinds of filamentous fungi. We constructed an Ash2 deletion mutant (ΔAsh2) by using an Agrobacterium-mediated gene knockout method to investigate the function of the Ash2 gene in the mold Monascus purpureus. Lack of the Ash2 gene resulted in the formation of a lower colony phenotype with fluffy aerial hyphae that autolyzed as the colony grew on potato dextrose agar at 30°C. The production of pigments and the number of conidia were significantly lower in the ΔAsh2 than in the wild type. Citrinin production by the ΔAsh2 was not detected during 15 days of fermentation. Relative expression levels of secondary metabolite regulatory genes PigR and CTNR, secondary metabolite synthesizing genes PKSPT and CTN, key genes of mitogen-activated protein kinase pathway Spk1 and its downstream gene mam2, the conidium development control gene BrlA, and global regulatory genes LaeA and VeA were detected by the quantitative real-time PCR. These results indicate that the Ash2 gene is involved in conidial germination, pigment production, and citrinin production and plays a key role in development and secondary metabolism in M. purpureus. HIGHLIGHTS Copyright ©, International Association for Food Protection.ABSTRACT Arsenic contamination in chicken is a cause of concern because of the potential health problems that may arise. In Trinidad and Tobago, chicken consumption averages about 53.8 kg per person per year, and chicken accounts for 85% of all the meat consumed locally. Fourteen chicken samples of imported, locally grown, and local organically farmed chickens were obtained from poultry depots and local supermarkets and were analyzed in triplicate. The concentration of total arsenic in the imported chicken ranged from below the method detection limit (MDL) to 512.50 μg/kg, and the concentration in the locally produced chicken ranged between less then MDL and 516.44 μg/kg. The estimated concentration of inorganic arsenic (iAs) in the imported chickens ranged between less then MDL and 333.12 μg/kg, whereas in local chicken it ranged between less then MDL and 335.68 μg/kg. The estimated daily intake (EDI) of iAs varies between 0 and 0.72 μg/kg/day. Five of the samples evaluated exceeded the tolerable limit of 0.