Activity

Silver ions, as a commonly used industrial heavy metal, tends to deposit in the body and induce many diseases. In this work, modified CdTe QDs with green and red emission were synthesized to assemble dual-QDs, which could be efficient and selective utilized for Ag+ determination through the electron transfer progress between surface functional group of dual-QDs and Ag+, and the aggregation of Ag+ on the surface of dual-QDs. Under the appropriate pH value and volume ratio, the interaction between the surface functional groups of assembled dual-QDs reduce the affinity of Hg2+ in this system. The fluorescent signal of dual-QDs simultaneously attenuation or enhancement in the same proportion remove the interference of Cu2+ and other metal ions. Therefore, this method can selectively detect Ag+ without any masking agents. The linear region of detection was from 0 to 800 nmol/L (R2 > 0.998), and low of detection (LOD) was 7.7 nmol/L, which could meet the corresponding standards of World Health Organization (WHO) and Environmental Protection Agency (EPA). This effective proposed dual-QDs ratios fluorescent probe has been applied to detect Ag+ in real environment water, tea and Citri Reticulatae Pericarpium (CRP) water. A novel solvothermal process for synthesizing InNbO4 nanomaterials was developed. In this manner, a series of InNbO4 samples was synthesized. It was shown that reaction temperature and precursor pH had strong influence on the attributes of InNbO4 samples. The X-ray diffraction patterns revealed that all the samples possessed monoclinic structure and the optimal reaction condition was found at 250 °C with a pH of 5. Scanning electron microscopy images of different InNbO4 samples showed various morphologies. Transmission electron microscopy verified the synthesized InNbO4-pH 5 was single-crystal cubes. X-ray photoelectron spectra verified the existence of In, Nb, and O in InNbO4-pH 5 sample. The band gap of InNbO4-pH 5 was calculated to be 2.51 eV. The photocurrent intensity of InNbO4-pH 5 was the highest among the prepared samples. The photocatalytic degradation of pefloxacin was investigated using these samples. The InNbO4-pH 5 exhibited best degradation efficiency among these samples. The removal efficiency of pefloxacin with InNbO4-pH 5 could reach 80.2% in 60 min. Based on free radical capture results, superoxide radicals and holes showed to be the dominant active species. In addition, UHPLC/MS/MS was used to identify the degradation intermediates. Five new pefloxacin degradation products were found and possible degradation pathways were suggested. V.The secondary metabolites produced by Fusarium can cause disease and death when consumed and produce biological responses even in the absence of the microorganism. The IL-6, TNF-α and TGF-β1 cytokines immune reactivity was associated with histopathological and physico-chemical changes in skin of immune competent rats after administration of Fusarium oxysporum crude extract. Rats were intradermally injected with 50 μl of 0.5 mg/ml crude extract and were euthanized at 3, 6, 12 and 24 h after injection. The inflammatory response was quantified by enzyme myeloperoxidase activity and by immunohistochemical method to detect the IL-6, TNF-α and TGF-β1. Physico-chemical analysis was performed using FT-Raman Spectroscopy. PFI-3 datasheet The inflammatory response was most intense at 6 and 12 h after crude extract administration and the most significant histopathological changes were observed in the dermis. Myeloperoxidase activity was intense from 3 to 24 h after injection. The immunostaining of pro-inflammatory cytokines IL-6 and TNF-α peaked at 6 h. Immunostaining for TGF-β1 was highest at 12 and 24 h. FT-Raman spectral analysis showed both, the most intense Fusarium interaction with the skin at 6 h, as revealed by the changes in the stretching of -CH bands (3100-2800 cm-1) in the dermis, and skin recovery trending after 12 h after crude extract injection. The results showed that secondary metabolites stimulated histopathologic changes and inflammatory responses even in the absence of the fungus, increasing myeloperoxidase activity and pro-inflammatory cytokine expression besides promoting physico-chemical changes. V.Magnesium isoglycyrrhizinate (MgIG) is the magnesium salt of 18β-glycyrrhizic acid extracted from licorice, a Chinese traditional medicine. The pharmacokinetic characteristics of MgIG have been widely studied; nevertheless, its target protein and mechanism of action remain unclear. Therefore, the objective of present work was to determine the characteristics of binding between human serum albumin (HSA) and MgIG. The formation of HSA-MgIG complex was studied using spectrometric techniques, LC-MS/MS, and molecular docking calculations. The results of fluorescence study demonstrated the quenching mechanism is definitely static. The negative thermodynamic parameters suggested that the interaction is enthalpically driven and occurs spontaneously. Binding density and probe displacement analysis suggested that MgIG bound to HSA at a single site, determined to be site I. The mean albumin binding rate of MgIG with HSA concentration ranged from 35 to 50 g·L-1 reached 85.6%. Molecular docking analysis revealed the major residues and interaction forces involved in formation of HSA-MgIG complex, corresponding with the experimental results. V.Co-crystals, which are defined as “solids that are crystalline materials composed of two or more molecules in the same crystal lattice” have recently been the focus of increased interest in the pharmaceutical industry since co-crystallization can improve unfavorable physicochemical properties of active pharmaceutical ingredients. Thus, the quest for new co-crystal screening methods has become an issue of importance. The aim of this work was, therefore, to show to what extent expanded methodology based on FTIR and Raman spectroscopy supported by the DSC method can be used as a reliable tool to screen co-crystallization. Because co-crystals of benzodiazepines had not yet been obtained, a set of 72 binary mixtures composed of eight 1,4-benzodiazepine derivatives and nine coformers were used as model substances. Potential co-crystals were prepared in solid-state by liquid-assisted grinding procedure. The characteristic FTIR and Raman bands which reflect hydrogen bond formation between benzodiazepine and coformer were used as proof of co-crystal creation.